穿心莲内酯在动物体内抗血小板凝聚的实验研究  

In vivo anti-platelet aggregation effects of andrographis paniculta lactone

作　　者：冯玉丽[1] 占葆娥[1] 张书楠[1] 李秀玉[1] 侯智辉[1] 袁敏[1] 

机构地区：[1]广东省深圳市中医院,广东深圳518033

出　　处：《国际检验医学杂志》2013年第4期385-386,共2页International Journal of Laboratory Medicine

基　　金：广东省建设中医药强省项目(2010307)

摘　　要：目的对穿心莲内酯在动物体内的抗血小板凝聚作用进行分析,证实其确切的作用,为临床用药提供参考。方法选取实验室养殖的健康大鼠30只为研究对象,将其随机分为3组:(1)对照组,服用定量的生理盐水;(2)观察1组,按50mg/kg的剂量服用穿心莲内酯;(3)观察2组,按100mg/kg的剂量服用穿心莲内酯。使用血小板凝聚仪及血栓形成仪对各组研究对象的血小板最大凝聚率和血栓形成时间进行测定,并进行比较及统计学分析。结果观察1组、观察2组研究对象的血小板最大凝聚率及血栓形成时间均较对照组有明显优势,经统计学分析,差异具有统计学意义(P<0.05);对观察1组和观察2组的血小板最大凝聚率及血栓形成时间进行组间比较,观察2组有明显优势,经统计学分析,差异具有统计学意义(P<0.05)。结论穿心莲内酯对大鼠体内的血小板凝聚及血栓形成具有显著的抑制作用,这种抑制作用随药物的剂量增加而增强。Objective To analyze the anti-platelet aggregation effects of andrographis paniculta lactone in animal body and con-firm the exact role to provide reference for clinical medication. Methods 30 healthy rats fed in laboratory were selected and random- [y divided into three groups,including control group fed quantitative physiological saline,observatio~ group 1 fed andrographis pani- culta laetone for 50 mg/kg and observation group 2 fed andrographis paniculta lactone for 100 mg/kg. Using platelet aggregation and thrombosis instrument to detect the largest platelet aggregation rate and thrombosis time,and comparison and statistical analy-sis were performed. Results The largest platelet aggregation rate and thrombosis time in observation group 1 and 2 were statistical-ly different with control group（P〈0.05） and also with statistical difference between the two observation groups（P〈0.05）. Con-clusion Andrographis paniculta lactone could significantly inhibit the platelet aggregation and thrombosis in rats,and this effects could be enhanced with the increasing of the drug dosage.

关 键 词：穿心莲内酯 血小板聚集 实验室技术和方法 

分 类 号：R285.5[医药卫生—中药学]