地黄饮子加减方对MCAO模型大鼠血清HPA轴及其脑组织细胞凋亡表达的干预效应研究  

Research on Dihuang Yinzi Decoction’s neuroprotection effects on MCAO rats from the HPA axis reaction and apoptosis aspects

作　　者：唐璐[1] 孙塑伦[1] 高颖[1] 朱陵群[2] 

机构地区：[1]北京中医药大学东直门医院神经内科,100700 [2]北京中医药大学东直门医院中医脑病研究室,100700

出　　处：《环球中医药》2013年第1期8-11,共4页Global Traditional Chinese Medicine

基　　金：国家科技重大专项"重大新药创制"(2009ZX09502-028);北京中医药大学科研基地建设项目(2011-JDJs-04);北京中医药大学创新团队项目(2011-CXTD-22)

摘　　要：目的探讨地黄饮子加减方对MCAO模型大鼠血清HPA轴含量及其脑组织细胞凋亡表达的干预效应。方法 MCAO法制作SD大鼠脑缺血模型,造模成功后第二天开始给予地黄饮子加减方浓缩煎剂灌胃,对照组灌胃等量的生理盐水,连续7天,于最后一次灌胃后1小时取材,放射免疫法检测血清HPA轴含量,TUNEL免疫组化法检测脑组织细胞凋亡表达。结果经地黄饮子治疗后:MCAO模型大鼠血清COR含量明显低于正常组、模型组,差异有显著统计学意义(P<0.01),ACTH、CRH含量均明显高于模型组,差异有显著统计学意义(P<0.05),缺血侧脑组织细胞凋亡表达较模型组明显减少,差异有显著统计学意义(P<0.01)。结论地黄饮子加减方能够通过纠正HPA轴的紊乱状态、抑制脑组织细胞凋亡表达等作用机制在脑缺血急性期发挥减轻脑损伤、改善预后的作用。Objective To explore the Dihuang Yinzi Decoction＇ s neuroprotection effects on MCAO rats from the HPA axis reaction and apoptosis aspects. Methods 30 SD rats were randomly divid- ed into 3 groups： MCAO model group, herb formula group, and normal group, each group was 10. For drug treatment, herb formula group were given Dihuang Yinzi Decoction from 2 days to 8 days after ische- mia by intragastric administration method, whereas the MCAO model group and normal group received e- quivalent volumes of distilled water. SD rats were killed 1 h after the last intragastric administration. The expression levels of ACTH, CRH, COR were detected by radioimmunoassay. The apoptosis of neuron was detected by TUNEL Immunohistochemistry. Results The level of COR in herb formula group were signifi- cantly lower than that in normal group and MCAO model group（P 〈0.01 ） ; The level of ACTH, CRH was significantly higher in herb formula group than in normal and MCAO model group（ P 〈 0.05 ） ; The expres- sion of apoptosis in neuron was significantly lower in herb formula group than in MCAO model group （ P 〈 0.01 ）. Conclusion Dihuang Yinzi Decoction may exert neuroprotection mechanism and improve the prognosis through restoring the disturbed condition of HPA axis reaction and inhibiting apoptosis.

关 键 词：地黄饮子 补肾护脑 MCAO模型大鼠 HPA轴 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]