霍乱弧菌O1胶体金免疫层析快速检测法的建立  

Development of Gold-immunochromatography Test for Rapid Detection of Vibrio Cholerae Group O1

作　　者：王玉金[1] 杨书豪[1] 刘丽[1] 庞向宇[1] 孙晨阳[1] 李珊珊[1] 

机构地区：[1]河南省科学院生物研究所有限责任公司,河南郑州450008

出　　处：《标记免疫分析与临床》2013年第1期36-39,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的建立一种快速、简易的检测霍乱弧菌O1群的胶体金免疫层析法(GICA)。方法利用胶体金免疫层析技术,采用双抗体夹心法检测霍乱弧菌O1群,对该法进行敏感性、特异性和稳定性分析。与细菌培养法对比检测208份临床标本。结果该法能在10分钟内完成检测;该试纸条仅与霍乱弧菌O1群阳性样品发生特异性反应;检测霍乱弧菌O1群的最低检出浓度为1×105cfu/mL;与细菌培养法对比检测208份临床标本,特异性和灵敏度均达100%。结论新建立的霍乱弧菌O1群胶体金免疫层析试验简便、快速,特异性和灵敏度较好,适用于现场样品的快速筛查。Objective To develop a rapid and simple colloidal gold immunochromatographic ssay （GICA） for the detection of Vibrio cholerae group O1. Methods A colloidal gold immunochromatography test based on doub- le antibody sandwich assay for detecting Vibrio cholerae group O1 was developed. Its sensitivity, specificity and stability were then evaluated. A total of 208 clinical specimens were determined with the prepared GICA, and the results were compared with those by bacterial culture. Results Typical detecting time was less than 10 minutes per sample. The positive samples produced a positive signal on immunochromatographic strip, whereas the negative one did not. The sensitivity of the test was 1 x10^5 cfu/ml. Both the specificity and sensitivity 6f the prepared GICA in determination of 208 clinical specimens were 100%. Conclusion The gold-immunochroma- tography test appears to be a rapid, convenient, specific and sensitive test for detecting Vibrio cholerae group O1 on site.

关 键 词：霍乱弧菌O1群 胶体金 免疫层析试验 快速筛查 

分 类 号：R817.4[医药卫生—影像医学与核医学]