RP-HPLC法测定Beagle犬血浆中的烟酸浓度及药动学研究  

Determination of niacin in dog plasma by RP-HPLC and its pharmacokinetics study

作　　者：赵孝蕊[1] 冯芳[1] 邹玲玲[1] 

机构地区：[1]中国药科大学药物分析教研室,南京210009

出　　处：《海峡药学》2013年第1期271-273,共3页Strait Pharmaceutical Journal

摘　　要：目的建立反相高效液相色谱法测定犬血浆中烟酸的浓度，并应用到烟葺盘缓释片在Beagle犬体内的药动学研究。方法采用高氯酸沉淀蛋白处理血浆样本；色谱条件：MegresC18柱（4．6mm×250mm，5μm），流动相：甲醇-10mmol·L-1磷酸二氢钾缓冲溶液（3：97，v／V），柱温；12℃。流速：0．8ml·min^-1，检测波长：263nm；比格犬灌胃蛤药500mg烟酸缓释片。结果线性范围为0．200-35．0ug·mL^-1（r≥0．9996），检测限为50ng·mL^-1，方法回收率为98．4％；烟酸缓释片表现出明显的缓释效果。结论方法操作简单、专属灵敏、重现性好，适用于Beagle犬体内烟馥的药动学研究。OBJECTIVE To establish a RP-HPLC method for the determination of niacin in dog plasma and the applied to a pharmacokinetics study of sustained-release（SR） niacin tablet in beagle dogs. METHEDS Sample pretreatment procedures involved protein precipitation by 14 % perchloric acid. Separation was performed on a Megres C18 column （4.6mm× 250mm, 5μm） and methanol-10mmol. L-1 KH2PO4 buffer （pH 2.6） with the ratio of 3：97（V/V） as the mobile phase at a flow rate of 0.8mL.min-1. The column temperature was 12℃ and the detection wavelength of263nm. Beagle dog was given 500mg SR niacin tablet by oral administration. RESULTS The linear range was 0.200 N 35.0μg.mL^-1 with r≥0.9996. The limit of determination was 50ng.mL-1. The mean recovery of niacin was 98.4 %. SR niacin tablet showed obvious sustained release effect. CONCLUTION The present RP-HPLC method was specific, simple, sensitive and applicable for the pharmaceutical study of niacin in beagle dogs.

关 键 词：烟酸缓释片 犬血浆 药代动力学 反相高效液相色谱 

分 类 号：R969.4[医药卫生—药理学]