不同加工方法杜梨叶中鞣质的含量测定  

Determination of tannin content in leaves of Pyrus betulaefolia Bge. with different processing methods

作　　者：王瑞华[1] 侯彩婷[1] 邹昀员[1] 田易玲[1] 刘玉红[1] 徐凌川[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《山东科学》2013年第1期47-50,64,共5页Shandong Science

摘　　要：采用磷钼钨酸-干络素法对不同加工方法杜梨叶中鞣质的含量进行测定。室温浸提过夜提取鞣质,在磷钼钨酸与多酚类物质的显色反应进行到90～120 min时,于760 nm波长处,分别测定供试样品溶液中的总酚和不被吸收的多酚的吸光度,以吸光度之差用标准曲线法求得鞣质含量。在1～10μg/mL范围内,线性关系良好(r=0.997 9)。平均回收率为98.58%(n=6),RSD为0.51%。此条件下,未发酵的杜梨叶鞣质平均含量在0.60%,渥堆发酵的杜梨叶未检出鞣质。We employ phosphorus molybdenumtungsten acid casein method to determine tannin content of two different products from the leaves of Pyrus betulaefolia Bge.. Tannins were extracted to be soaking at room temperature by staying overnight When the color reaction between phosphomolybdium Tungslic acid and phenolic acids was carrying through 90- 120 min, absorbency of tolal phenolic acids and unabsorbable phenolic acids was delermined respectively at The wavelength of 760 nm. Tannins content is derived by spectrophotometry difference and standard curve method. Better linear relationship （ r=0. 997 9） exists in the range of 1 - 10 μg/mL. We obtain average recovery rate of 98. 581% （ n =6） and RSD of 0.51%. Tannins average content of unfermented leaves is 0.60%, and tannins is not detected in the leaves of fermentation under this condition.

关 键 词：杜梨叶 磷钼钨酸-干酪素法 鞣质 含量测定 

分 类 号：R284[医药卫生—中药学]