中草药对刺参腐皮综合症病原菌的抑菌试验  

In vitro Bacteriostatic Effect of Chinese Herbs against Causative Pathogens of Skin Ulcer Syndrome in Sea Cucumber Apostichopus japonicus

作　　者：侯文久[1] 杨凤影[1] 王维新[1] 

机构地区：[1]辽宁医学院畜牧兽医学院,辽宁锦州121001

出　　处：《中国农学通报》2013年第5期76-80,共5页Chinese Agricultural Science Bulletin

基　　金：辽宁省农业科技攻关项目"刺参无公害免疫抗菌物质的筛选及绿色新型饲料的开发与应用"(2011211002)

摘　　要：为探讨中草药对刺参"腐皮综合症"的治疗效果,用抑菌圈法和改良2倍稀释法测定了42种单方中草药和9种复方中草药对刺参"腐皮综合症"病原菌——黄海希瓦氏菌的体外抑菌活性。结果显示:诃子、五倍子、牡丹皮、石榴皮、金樱子、地榆、丹参和丁香8种草药的效果较好。将诃子、石榴皮、丹参、丁香4种单方中草药组成9种复方,复方最低抑菌浓度显著好于单方,最低最低抑菌浓度为0.547mg/mL。试验结果显示:复方最佳配比为诃子:石榴皮:丹参:丁香=2:1:1:1。In order to study the treatment effect of Chinese herbs to skin ulcer syndrome in sea cucumber.The bacteria static effect of 42 kinds of Chinese herbs and 9 kinds of herb compounds were determined in vitro by bacterio static circle and improved micro dilution procedure.This work conducted as the herbs were against one of causative pathogens(Shewanella marisflavi) of skin ulcer syndrome in sea cucumber Apostichopusjaponicus.The results illustrated that 8 kinds of Chinese herbs(Terminaliachebuia,gallnut,Cortex Moutan,Pericarpium Granati,Rosa laevigata,sanguisorba,Radix Salviae Miltiorrhizae and clove) had better bacteria static effect on the pathogen.Nine herbal compounds were composed by 4 herbs,the bacterio static effect of 9 compounds enhanced remarkably than single herbs,and the lowest MIC was 0.547 mg/mL.According to the bacterio static effects,the best ratio of the compounds was determined as Terminaliachebuia:Pericarpiumgranati:Radix salviae miltiorrhizae:clove=2:1:1:1.

关 键 词：刺参 腐皮综合症 中草药 最低抑菌浓度 配方 

分 类 号：S9[农业科学—水产科学]