RP-HPLC法同时测定淫桂通便颗粒中6种成分  

Determination of six components in Yingui Tongbian Granules by RP-HPLC

作　　者：王蕾[1] 谈瑄忠[2] 毛春芹[1] 陆兔林[1] 王会娟[1] 胡颖菲[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]南京市中医院,江苏南京210001

出　　处：《中成药》2013年第2期293-296,共4页Chinese Traditional Patent Medicine

基　　金："十二五"南京市医学科技发展重大项目(2012年)

摘　　要：目的建立同时测定淫桂通便颗粒(肉桂、淫羊藿、厚朴、葛根、薤白、茯苓)中葛根素、淫羊藿苷、肉桂酸、肉桂醛、和厚朴酚与厚朴酚的反相高效液相方法。方法 Kromasil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液梯度洗脱,体积流量1.0 mL/min,柱温35℃,检测波长290 nm。结果通过方法学考察,葛根素在1.56～7.80μg、淫羊藿苷在8.70～43.50μg、肉桂酸在7.37～36.84μg、肉桂醛在0.50～2.50μg、和厚朴酚在8.92～44.62μg、厚朴酚在11.14～55.69μg范围内,均成良好的线性关系;相关系数分别为0.999 9、0.999 6、0.999 7、0.999 8、0.999 7、0.999 7;平均回收率分别为97.20%、98.78%、97.48%、97.39%、98.81%、98.40%,RSD分别为2.15%、0.79%、1.71%、1.47%、0.82%、0.91%。结论该方法简便、准确、重复性好,可作为该制剂的质量控制。AIM To develop a RP-HPLC method for determining puerarin, icraiin, cinnamic acid, cinnamal dehyde, honokiol and magnolol in Yingui Tongbian Granules （ Cinnamomi Cortex, Epimedii Folium, Magnoliae of-ficinalis Cortex, Puerariae lobatae Radix, Allii macrostemi Bulbus , Poria ） . METHODS Kromasil Cls （250 mm x 4.6 ram, 5 μm） column was used with the mobile phase consisted of acetonitrile -0. 1% phosphoric acid in gradient elution at the flow rate of 1.0 mL/min. The column temperature was maintained at 35 .C and the detection wavelength was set at 290 nm. RESULTS The methodological study showed that the linear ranges of puerarin, icraiin, cinnamic acid, cinnamaldehyde, honokiol and magnolol were 1.56-7. 80 μg （r = 0. 999 9）, 8.70-43.50μg （r=0.9996）, 7.37-36.84 μg （r-0.9997）, 0.5-2.5 μg （r=0.999 8）, 8.92-44.62 μg （r=0.9997） and 11.14-55.69 μg （ r = 0. 999 7 ）, respectively. The average recoveries were 97.20% , 98.78% , 97.48% , 97.39%, 98.81% and 98.40%, respectively. RSDs were 2. 15%, 0.79%, 1.71%, 1.47%, 0.82% and 0. 91% , respectively. CONCLUTION The method is sample, accurate and reproducible, and can be used for the quality control of the preparation.

关 键 词：淫桂通便颗粒 葛根素 淫羊藿苷 肉桂酸 肉桂醛 和厚朴酚 厚朴酚 HPLC 

分 类 号：R927.2[医药卫生—药学]