射黄含片的质量标准研究  

Quality standard for Shehuang Lozenges

作　　者：田成旺[1,2] 郭建华[3] 张科[3] 张铁军[2] 蒋伶活[1,4] 

机构地区：[1]天津大学药物科学与技术学院,天津300072 [2]天津药物研究院,天津300193 [3]天津医科大学药学院,天津300070 [4]江南大学生物工程学院,江苏无锡214122

出　　处：《中成药》2013年第2期302-306,共5页Chinese Traditional Patent Medicine

基　　金：国家科技支撑计划项目(2006BAI06A01-02)

摘　　要：目的建立射黄含片(射干、黄芩)的质量标准。方法采用薄层色谱法对射黄含片中的射干和黄芩进行定性研究;采用高效液相色谱方法,选用DiamonsilC18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.05%磷酸溶液(42∶58),体积流量为1.0 mL/min,检测波长为270 nm,同时定量检测射黄含片中黄芩苷、射干苷和次野鸢尾黄素。结果定性鉴别专属性强;黄芩苷、射干苷和次野鸢尾黄素分别在进样量140～840 ng、40～240 ng和20～120ng范围内线性关系良好,r值均在0.999 8以上,平均加样回收率分别为98.89%(RSD为1.63%)、100.32%(RSD为1.06%)和98.65%(RSD为1.53%)(n=9)。结论该方法操作简便,结果准确,可用于射黄含片的质量控制。AIM To establish quality standard for Shehuang Lozenges （Belamcandae Rhizoma, Scutellariae Radix）. METHODS Scutellariae Radix and Belamcandae Rhizoma were identified by TLC method. Baicalin, tectoridin and irisflorentin in Shehuang Lozenges were determined by RP-HPLC simultaneously. The Diamonsil C18 （4.6 mm x250 mm, 5 μm） column was used. The mobile phase was methanol -0.05% phosphoric acid solution （42 ： 58） with the flow rate of 1.0 mL/min. The detection wavelength was 270 nm. RESULTS The qualitative identification was specific. Baicalin, tetoridin and irisflorentin showed good linear relationships in the ranges of 140-840 ng （r =0. 999 4）, 40-240 ng （r =0. 999 8） and 20-120 ng （r =0. 999 9）, respectively. Their average recoveries were 98.89% , 100. 32% and 98.65% , with RSDs of 1.24% , 1.06% and 1.53% （n = 9）, respectively. CONCLUSION The method is simple, accurate and reliable. It can be used for the quality control of the preparation.

关 键 词：射黄含片 薄层色谱 反相高效液相色谱 射干苷 黄芩苷 次野鸢尾黄素 

分 类 号：R927.2[医药卫生—药学]