黄芩苷诱导大鼠气管上皮细胞Mx1和PKR表达的研究  

On the baicalin-induced rat trachea epithelial cells expressing Mx1 and PKR

作　　者：崔永勇[1] 高立云[1] 张涛[1] 董虹[1] 段慧琴[1] 穆祥[1] 胡格[1] 杨佐君[1] 

机构地区：[1]北京农学院兽医学(中医药)北京市重点实验室,北京102206

出　　处：《北京农学院学报》2013年第1期19-21,共3页Journal of Beijing University of Agriculture

基　　金：国家自然科学基金青年基金项目(30500368);北京市教委人才强教深化计划-中青年骨干人才子项目(PHR201008422);北京市教委科技计划面上项目(KM201110020009);北京市科技新星A类项目(2006A26)

摘　　要：为了研究黄芩苷能否诱导大鼠气管上皮细胞表达抗病毒蛋白,以体外培养的大鼠气管上皮细胞(tracheaepithelial cells,TECs)为模型,采用Western Blotting方法检测了不同质量浓度(10、20、30μg/mL)和不同时间段(6、12、24h)黄芩苷诱导TECs表达抗病毒蛋白(AVPs)Mx1和PKR的量。结果表明,黄芩苷在3个浓度梯度和3个时间段均可以诱导TECs分泌抗病毒蛋白Mx1和PKR;并在30μg/mL,24h时使TECs所产生的抗病毒蛋白最高。这一结果为体外筛选诱导TECs表达抗病毒蛋白的中药有效成分提供了参考,为进一步研究中药抗病毒机理提供了实验依据。In order to understand whether baicalin could induce rat trachea epithelial cells （TECs） to express anti-viral protein （AVPs）, this experiment set TECs as a model in vitro and detected the amount of the anti-viral protein （Mxl, PKR） expressed by TECs which is induced by baicalin at different concentrations （ 10, 20 and 30μg/mL ） for different periods （ 6, 12 and 24 h ） by western blotting. The results showed that baicalin could successfully induce TECs to secrete anti-viral protein Mxl and PKR at three concentrations for three periods; and TECs secreted the most anti-viral protein at the concentration of 30 /lg/mL in 24 h. This result provides a reference for screening the effective component of traditional Chinese medicine which induces TECs to express the anti-viral protein in vitro as weI1 as studying on the anti-viral mechanism of traditional Chinese medicines.

关 键 词：黄芩苷 气管上皮细胞 抗病毒蛋白 western BLOTTING 大鼠 

分 类 号：S853.74[农业科学—临床兽医学]