乙酰化白藜芦醇通过调节缝隙连接蛋白43表达减轻海水淹溺型急性肺损伤  

Acetylated resveratrol alleviates seawater drowning induced acute lung injury by enhancing the expression of Connexin43

作　　者：马李杰[1] 李艳燕[1] 赵诣林[1] 刘雪英[2] 谢永宏[1] 梁力[2] 南岩东[1] 穆德广[1] 李王平[1] 金发光[1] 

机构地区：[1]第四军医大学唐都医院呼吸内科,陕西西安710038 [2]第四军医大学药学系药物化学教研室,陕西西安710032

出　　处：《中华肺部疾病杂志（电子版）》2013年第1期10-12,16,共4页Chinese Journal of Lung Diseases(Electronic Edition)

基　　金：2012年国家自然科学基金面上项目(81270124)

摘　　要：目的观察海水淹溺肺损伤大鼠肺组织中缝隙连接蛋白43(Cx43)的表达变化及乙酰化白藜芦醇对其的干预作用。方法 32只大鼠完全随机分为空白对照组、模型组、乙酰化白藜芦醇预处理组和乙酰化白藜芦醇组,每组8只。采用气管内滴注海水(4 ml/kg)的方法制作海水淹溺型急性肺损伤大鼠模型,用ELISA法检测肺组织中TNF-α和IL-10的含量,实时定量PCR及免疫组化的方法检测肺组织中Cx43的表达变化。结果吸入海水4 h后,大鼠肺部损伤明显,TNF-α和IL-10的含量增高,Cx43在基因水平表达量增高,而蛋白水平表达降低;乙酰化白藜芦醇预处理明显减轻海水吸入导致的急性肺损伤,减少炎症因子分泌并且在基因水平和蛋白水平增加Cx43的表达。结论 Cx43参与海水淹溺型急性肺损伤的发生与发展,乙酰化白藜芦醇能够通过增加Cx43的表达减轻肺损伤。Objective To observe the expression of Connexin43 （Cx43） in rat lungs suffered from seawater inhalation and the intervention effect of acetylaled resveratrol. Methods 32 rats were randomly divided into normal control group, model group, acetylated resveratrol pre-treated group, and acetylated resveratrol group. Seawater drowning induced acute lung injury （SWD-ALI） model was made by instillation of seawater in to air way. Pathology detection was carried out on lungs four hours after modeling, ELISA was used to measure the contents of TNF-α and IL-10, and the expression of Cx43 in lungs was detected by using real- time PCR and immunohistochemistry. Results Obvious lung injury was detected 4 hours after seawater instillation, contents of TNF-α and IL-10 increased, and the expression of Cx43 was up-regulated on gene level but decreased on protein level. While pre-treatment of acetylated resveratrol attenuated lung injury, suppressed the secretion of inflammatory factors, and increased the expression of Cx43 on gene and protein level. Conclusions Cx43 plays part in occurrence and development of SWD-ALI, and acetylated resveratrol can attenuate ALI by enhancing the expression of Cx43.

关 键 词：急性肺损伤 海水淹溺 乙酰化白藜芦醇 缝隙连接蛋白43 

分 类 号：R563[医药卫生—呼吸系统]