消栓通脉颗粒药物血清对TNF-α诱导人脐静脉内皮细胞NF-κB表达的影响  

TNF-α Inducting Expression of Human Umbilical Vein Endothelial Cells NF-κB and Intervention of Chinese Herbal Medicine

作　　者：阎伟[1,2] 白增亮[1] 侯玉芬[2] 

机构地区：[1]山东大学生命科学院,发育免疫学济南250011 [2]山东中医药大学附属医院,济南250011

出　　处：《中国中西医结合外科杂志》2013年第1期40-42,共3页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：山东省自然基金课题(Y2008C148)

摘　　要：目的:研究核转录因子NF-κB在TNF-α诱导的人脐静脉血管内皮细胞中的表达,探讨NF-κB在DVT炎症-血栓反应环节的作用及中药消栓通脉颗粒的干预作用。方法:以TNF-α诱导人脐静脉内皮细胞为实验模型,分为模型组、消栓通脉高剂量组、中剂量组及低剂量组,运用RT-PCR、Western-Blot方法检测NF-κB表达量的变化。结果:人脐静脉内皮细胞经TNF-α刺激后,NF-κB的表达水平比未激活的细胞中NF-κB水平明显升高。消栓通脉颗粒含药血清干预后,NF-κB表达水平下降,且该抑制作用呈剂量依赖效应,高剂量含药血清作用下,NF-κB表达水平最低。结论:消栓通脉颗粒能够抑制激活的人脐静脉血管细胞中NF-κB的表达。objective To research the expression of nuclear transcription factors NF-κB in the TNF-α induction of human umbilical vein endothelial cells in the link of reaction function and Xiao Shuan and to evaluate the NF-κB in DVT inflammation-blood clots Tong Mai （消栓通脉） intervention role. Methods With TNF-α induction human umbilical vein endothelial cells as experimental models, thirty rats were divided into the model group, Xiao Shuan Tong Mai（消栓通脉）high dose group, middle dose group and low dose group, The quantity changes of the NF-κB expression. RT-PCR and Western-Blot methods were used to detect. Results In human umbilical vein endothelial cells after TNF-α stimulation, the NF-κB expression level increased significantly higher than the cells unactivated NF-κB level. Under the role of serum containing Xiao Shuan Tong Mai （消栓通脉）, NF-κB expression level dropped, and this inhibition was dose dependent effect. Under the role of high serum dose medication, the NF-κB expression level was minimal. Conclusion Xiao Shuan Tong Mai（消栓通脉）can inhibit the expression of NF-κB in activated human umbilical vein endothelial cells.

关 键 词：人脐静脉血管内皮细胞 核转录因子-ΚB 消栓通脉颗粒 

分 类 号：Q95-33[生物学—动物学] R285.5[医药卫生—中药学]