好氧和厌氧条件下Acidithiobacillus ferrooxidans菌对Fe^(3+)氧化FeS_2的影响  

Effect of Acidithiobacillus ferrooxidans on pyrite oxidation by ferric iron under aerobic and anaerobic conditions

作　　者：刘欣伟[1] 冯雅丽[1] 李浩然[2] 杨志超[1] 蔡震雷[1] 

机构地区：[1]北京科技大学土木与环境工程学院,北京100083 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190

出　　处：《中国有色金属学报》2013年第1期274-281,共8页The Chinese Journal of Nonferrous Metals

基　　金：国家自然科学基金资助项目(20876160;21176026);中央高校基本科研业务费专项资金资助项目(FRT-TP-09-002B)

摘　　要：嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans:A.ferrooxidans菌)是目前研究得最多的浸矿细菌,其能量代谢途径复杂多样。在好氧和厌氧气氛下,分别对Fe3+浸出黄铁矿及A.ferrooxidans菌对Fe3+氧化浸出黄铁矿的影响进行了研究,并且利用A.ferrooxidans菌构建微生物燃料电池,研究在不同气氛下A.ferrooxidans菌对电子传递过程的影响。结果表明:在好氧和厌氧气氛下,加菌时的黄铁矿浸出率比无菌时的分别提高了40.03%和27.76%。在好氧和厌氧气氛下,A.ferrooxidans菌均可以提高电子传递速率,进而加快氧化还原反应的进行,说明A.ferrooxidans菌在厌氧环境下,能以Fe3+为电子受体、含还原性硫的黄铁矿为电子供体来进行呼吸作用,获得生命活动所需的能量。在实验结果和前人工作的基础上提出在厌氧情况下,A.ferrooxidans菌进行呼吸作用的一条可能的路线图。Acidithiobacillusferrooxidans （A. ferrooxidans） is the most widely studied bacteria, which is used in leaching ores work. The pyrite leached by ferric iron and the effect ofA. ferrooxidans on pyrite leaching were studied under the aerobic and anaerobic atmosphere, respectively. Bio-cathode microbial fuel cells were constructed in accordance with the characteristics of A. ferrooxidans, and then used to study the effect of A. ferrooxidans on the electron transfer process under the aerobic and anaerobic atmosphere. The results show that, under the aerobic and anaerobic atmosphere, the leaching rates of pyrite are 40.03% and 27.76% more than those of chemical leaching, respectively. Under the aerobic and anaerobic atmosphere, the presence of A. ferrooxidans both can improve the electron transfer rate, and thus speed up the conduct of the redox reaction. This demonstrates that, in the anaerobic environment, A. ferrooxidans can be respiration using Fe3＋ as electron acceptor and pyrite containing reduced sulfur as electron donor. On the basis of experimental results and previous work, a possible electron transport chain ofA. ferrooxidans under anaerobic condition was proposed.

关 键 词：嗜酸氧化亚铁硫杆菌 铁氧化系统 硫氧化系统 电子传递 

分 类 号：Q935[生物学—微生物学]