PvMSP1-19重组蛋白体外对间日疟患者外周血T细胞免疫功能的影响  

Immune effect of T lymphocytes from Plasmodium vivax-infected patients stimulated by recombinant PvMSP1-19 antigen in vitro

作　　者：李光友[1,2] 夏惠[1] 陶志勇[1] 陈勇[1] 方强[1] 

机构地区：[1]蚌埠医学院病原生物教室,蚌埠233030 [2]安徽省芜湖市第二人民医院检验科,芜湖241001

出　　处：《中国人兽共患病学报》2013年第2期175-178,共4页Chinese Journal of Zoonoses

基　　金：安徽省高等学校自然科学研究重点项目(No.kj2012A200);卫生部寄生虫病预防与控制技术重点实验室开放课题(No.WK009-001)联合资助~~

摘　　要：目的探讨PvMSP1-19重组蛋白对间日疟既往感染者外周血T淋巴细胞功能的影响。方法间日疟既往感染者外周血单个核细胞(PBMC),在体外分别以PvMSP1-19重组蛋白和IL-2(刺激组)及IL-2(未刺激组)培养,培养第7d时刺激组细胞再用PvMSP1-19重组蛋白刺激24h,用流式细胞仪检测T淋巴细胞亚群分泌IL-4和IFN-γ的情况;并以CFSE标记法检测T淋巴细胞的增殖反应。结果在分泌IL-4的淋巴细胞中,CD8+T细胞所占的比例刺激组(8.04%)明显高于未刺激组(3.46%);而分泌IFN-(的CD8+T细胞在刺激组(0.82%)与未刺激组(1.05%)间并无显著性差别。分泌IL-4和IFN-(的CD4+T细胞比例在刺激组(1.81%,0.19%)与未刺激组(1.89%,0.05%)间并无显著性差异。此外,CD8+T细胞的增殖指数刺激组(5.65%)也明显高于未刺激组(3.69%)。结论 PvMSP1-19重组蛋白能显著诱导间日疟既往感染者外周血CD8+T细胞增殖和优先分泌IL-4。In order to study the effect of T cells from Plasmodium vivax （P. vivax）-infected patients which were stimu- lated with PvMSPI-19 in vitro, the peripheral blood mononuclear cells （PBMCs） isolated from P. vivax-infected patients was stimulated with PvMSPI-19 protein, and cultured in rIL-2 containing media to generate PvMSPI-19 activated T cells. After 7 days of culture, PvMSPl-19 was used to restimulate the activated T cells for 24 hours. Then the cells were collected for detec- ting intracellular cytokines by Flow cytometry （FCM）, and the cells-proliferations of T cells labeled by CSFE was also analysed by FCM. The percentages for IL-4＋ T cells among activated CD8＋ T cells that were stimulated with PvMSPI-19 was distinctly higher than that in the unrestimulated control group （P^0.05）. Meanwhile, PI （proliferative index） of CD8＋T cells that were stimulated with PvMSPI-19 was distinctly higher than that in the unrestimulated control group （P%0.05）. In conclusion, the recombinant PvMSPI-19 protein could effectively stimulate the proliferation of CD8＋ T cells and the production of IL-4 secreted by CDS＋T cells.

关 键 词：间日疟 PvMSP1-19 IL-4 IFN-y 

分 类 号：R382[医药卫生—医学寄生虫学]