不同剂量瑞舒伐他汀对ox-LDL诱导的人单核-巨噬细胞组织因子表达的影响及机制  

Effects and mechanism of different doses of rosuvastatin on expression of tissue factor in cultured human monocyte-macrophage cells induced by oxidized low density lipoprotein

作　　者：苗晓慧[1] 宋海彬[1] 申晓彧[1] 

机构地区：[1]山西医科大学第二临床医院心血管内科,太原030001

出　　处：《中国医师杂志》2013年第1期21-24,共4页Journal of Chinese Physician

基　　金：山西省科技攻关项目（20120313020-1O）

摘　　要：目的研究不同剂量瑞舒伐他汀对氧化修饰低密度脂蛋白（ox-LDL）诱导的人单核-巨噬细胞组织因子（TF）表达的影响及可能机制。方法实验分七组：空白对照组、ox-LDL对照组、多聚肌苷酸组、不同剂量（0．01、0．1、1、5μmol／L）瑞舒伐他汀组。RT-PCR检测各组血凝素样氧化低密度脂蛋白受体-1（LOX-1）mRNA、TFmRNA的表达水平，ELISA检测各组TF蛋白含量的表达。结果不同剂量瑞舒伐他汀对ox-LDL诱导后人单核-巨噬细胞LOX-1mRNA、TFmRNA和TF表达的影响：七组之间比较，差异均有统计学意义（F=91．334，58．833，103．552，P〈0．05）。OX-LDL组与空白组比较，LOX-1mRNA、TFmRNA、TF的表达均增加[（3．25156±0．15772）vs（1±0）；（2．522451±0．138967）vs（1±0）；（207．7233±1．154701）ng／L vs（184．8467±0．871799）ng／L]，差异有统计学意义（P〈0．05）；多聚肌苷酸组、各瑞舒伐他汀组与OX—LDL组比较，三者表达均下降，且不同剂量瑞舒伐他汀组表达呈剂量依赖性减少，差异均有统计学意义（P〈0．05）；不同剂量瑞舒伐他汀组之间进行两两比较时，各组间差异有统计学意义（均P〈0．05）。结论LOX-1介导的ox-LDL可使人单核．巨噬细胞TF表达增加，瑞舒伐他汀可通过ox-LDL途径，且呈剂量依赖性下调单核一巨噬细胞LOX-1mRNA、TFmRNA的表达，使TF蛋白表达减少。Objective To investigate the effects and mechanism of different doses of rosuvastatin on expression of tissue factor（TF） in cultured human monocyte-macrophage cells which were induced by ox- idized low density lipoprotein （ox-LDL）. Methods The human monocyte-macrophage cells were divided into seven groups ： control group, ox-LDL group, poly-insine monophosphate group, different doses of rosuvastatin group（0. 01 μmol/L, 0. 1 μmol/L, 1 μmol/L, 5 μmol/L）. The expression of LOX-1 mRNA and TF mRNA was assayed by RT-PCR. The enzyme-linked immunosorbent assay was performed to determine the protein concentration of TF. Results Effects of different doses of rosuvastatin on expressions of LOX-1 mRNA, TF mRNA and TF in cultured human monocyte-macrophage cells induced by ox-LDL： comparison among seven groups, the difference was statistically significant （ F = 91. 334, 58. 833,103. 552, P 〈 0. 05 ）. Compared with control group, the expressions of LOX-1 mRNA, TF mRNA and TF were increased in the ox-LDL , group[ （3. 25156 ± 0. 15772） vs （I1±0） ; （2. 522451±0. 138967） vs （1 ±0） ; （207. 7233± 1. 154701 ）ng/L vs （184. 8467 ±0. 871799 ）ng/L ], and they were in a concentration-dependent manner （ P 〈 0.05 ）. Compared with the PolyI group and the different doses of rosuvastatin group, the expressions of LOX-1 mRNA, TF mRNA and TF were in the ox-LDL group, and the different doses of rosuvastatin were decreased by dose-dependent manner. It was in a concentration dependent manner （ P 〈 0. 05 ）. Different doses of rosuvastatin were compared between groups （ between each group P 〈 0.05 ） , the difference between each two groups was statistically significant （ P 〈0. 05）. Conclusions LOX-1 may be responsible for the expression of TF in Human monocyte-macrophage cells induced by ox-LDL. Rosuvastatin by dose d pendent manner and by means of ox-LDL reduced monocyte-macrophage LOX-1 mRNA and TF mRNA expressions, which reduced expression of TF.

关 键 词：氟苯类 药理学 嘧啶类 药理学 磺胺类 药理学 氟苯类 投药和剂量 嘧啶类 投药和剂量 磺胺类 投药和剂量 脂蛋白类 LDL 单核巨噬细胞系统 代谢 单核巨噬细胞系统 药物作用 凝血致活酶 代谢 

分 类 号：R543.4[医药卫生—心血管疾病]