异氟烷预处理对大鼠肝部分缺血再灌注时脑的保护作用  

Brain protection of isoflurane preconditioning on the rat fiver agaimt ischemia-reperfusion

作　　者：刘东俊[1,2] 吕金[1] 陈华[1] 沈洁[1] 

机构地区：[1]中国医科大学附属盛京医院麻醉科,沈阳110004 [2]辽宁省沈阳市妇婴医院麻醉科

出　　处：《中国医师杂志》2013年第1期25-28,共4页Journal of Chinese Physician

基　　金：辽宁省教育厅课题项目（2008792）

摘　　要：目的通过观察在体大鼠肝部分缺血再灌注损伤下外周血中S-100β蛋白含量的变化，结合脑组织线粒体形态学的改变明确异氟烷预处理对大鼠肝部分缺血再灌注时对脑损伤是否具有保护作用。方法SD大鼠简单随机法分成假手术组（s组），肝缺血再灌注组（I／R组），异氟烷预处理组（ISO组）。S组：入腹后只分离肝十二指肠韧带，但不阻断肝门血供；I／R组：肝缺血60min，再灌注120min；ISO组：肝I／R前60minISO预处理30min，后在空气中洗脱30min，再灌注24h迅速断头取前脑，透射电镜下观察脑线粒体超微结构；各组分别于缺血前及再灌注120min后抽取静脉血采用双抗体夹心．ELAISA法测定S-100β蛋白含量。结果I／R组超微结构脑线粒体肿胀，嵴断裂、溶解，内呈空泡状，线粒体形态及膜完整性发生了重大变化，外周血液S-100β蛋白I／R组[（1．52±0．26）μg／m1]显著高于对照组[（0．31±0．05）μg／m1]（P〈0．05）；ISO组线粒体仅有轻度肿胀，嵴及内膜间隙仍可见，S-100β蛋白ISO组[（0．79±0．21）μg／m1]明显低于I／R组（P〈0．05）。结论大鼠肝部分缺血再灌注后对脑组织造成了一定程度损伤，而异氟烷预处理对此具有一定保护作用。Objective To investigate the brain protective effect of isoflurane preconditioning on the rat liver against ischemia-reperfusion through determining the content of S-100β protein in peripheral blood in combination with mitochondrial ultrastructure in rat brain. Methods A total of 45 SD rats were randomly divided into three groups, sham group （group S） ： the only separation of the hepatoduodenal ligament, but did not block the hepatic portal blood supply; ischemia-reperfusion group （group I/R） ： liver ischemia 60 min, reperfusion 120 min; isoflurane preconditioning group （group ISO） ： 60 min before liver I/R, ISO pretreatment for 30 min, elution in the air after 30 min; 24 h after recirculation the forebrain tissues were rapidly removed. The changes of mitochondrial uhrastructure were observed by electron microscopy. The content of S-100β protein in serum was measured before ischemia and reperfusion 120 min through the ap- plication of Elisa kit. Results Marked swelling of mitochondria with disrupted cristae and damaged matrix were observed in group I/R, while relative intact mitochondria were seen in sham and ISO groups. The content of S-100β protein in serum was significantly higher in I/R group [ （1.52 ±0. 26）μg/ml] than in sham [（0.31 ±0. 05）μg/ml] and ISO [（0.79 ±0.21）μg/ml] groups （ P 〈0.05）. Conclusions The liver ischemia-reperfusion may injure the brain of the rat and isoflurane preconditioning can protect the rat brain from injury.

关 键 词：大鼠 Sprague-Dawley 疾病模型 动物 缺血预处理 异氟醚 药理学 肝 再灌注损伤 预防和控制 脑 药物作用 

分 类 号：R285.5[医药卫生—中药学]