应用双通道Taqman-MGB探针技术进行Kidd血型基因分型  

Genotyping JK^a and JK^b Alleles of KIDD Blood Group by Real Time PCR with Double Channel Taqman-MGB Probes

作　　者：秦斐[1] 何吉[1] 许先国[1] 洪小珍[1] 刘瑛[1] 陈舒[1] 朱发明[1] 吕杭军[1] 

机构地区：[1]浙江省血液中心输血研究所,卫生部血液安全研究重点实验室,杭州310006

出　　处：《医学研究杂志》2013年第2期81-84,共4页Journal of Medical Research

基　　金：浙江省医药卫生科学研究基金资助项目(2011RCB012)

摘　　要：目的探讨利用双通道Taqman-MGB探针检测Kidd血型系统JKa、JKb等位基因的可行性。方法基于JKa、JKb等位基因在SLC14A1基因838G>A的差异,设计引物和Taqman-MGB探针建立JKa、JKb等位基因分型方法,并将基因分型与血清学分型结果进行比较。结果双通道TaqMan-MGB探针实时荧光PCR方法能有效检测JKa、JKb等位基因。在198例随机样本中,JKa等位基因频率为0.427,JKb为0.573,基因分型结果与血清学分型结果完全一致。结论建立了同步检测JKa、JKb等位基因的双通道实时荧光PCR方法。Objective To explore the feasibility of genotyping for JK^a and JKb alleles of KIDD blood group by real time PCR （ QT - PCR） with Taqman - MGB probes. Methods Based on the difference of JK^aand JK^b alleles at position 838G 〉 A polymorphism in SLC14A1 gene, the amplification primers and Taqman -MGB probes were designed to establish the genotyping method to distinguish JKa and JK^b alleles. The results with genotyping were compared with that of serologic method. Results QT - PCR with double channel Taq- Man - MGB probes could distinguish JK^aand JK^b alleles effectively. The frequencies of JKa and JK^b alleles were 0. 427 and 0. 573 in the 198 individuals. The results with genotyping were consistent with that of serologic typing. Conclusion This study developed a QT - PCR with double channel TaqMan - MGB probes, which could simultaneously genotype for JK^a and JK^balleles.

关 键 词：KIDD血型 基因分型 实时荧光PCR 

分 类 号：R378.911[医药卫生—病原生物学]