临产后子宫体部平滑肌高表达HSP27和αB-crystallin  

HSP27 and αB-crystallin are highly up-regulated in corpus uteri myometrium at labor

作　　者：马薇[1] 周昌菊[2] 张卫社[3] 

机构地区：[1]西北民族大学医学院,甘肃兰州730030 [2]中南大学湘雅三医院妇产科,湖南长沙410013 [3]中南大学湘雅医院妇产科,湖南长沙410008

出　　处：《中国病理生理杂志》2013年第2期334-340,共7页Chinese Journal of Pathophysiology

基　　金：湖南省卫生厅科研基金项目(No.B2005-068);中央高校基本科研业务费专项资金资助项目(No.zyz2012091)

摘　　要：目的:研究临产前后子宫体部平滑肌热休克蛋白27(HSP27)和α-晶体蛋白B链(αB-crystallin)的表达变化,探讨其在临产后子宫平滑肌收缩中的可能作用。方法:采用比较蛋白质组学技术鉴定出足月未临产和自然临产人子宫体部平滑肌中HSP27和αB-crystallin蛋白存在差异性表达,应用RT-PCR、免疫组化和免疫印迹方法对临产前后子宫体部平滑肌中HSP27蛋白的表达进行验证。结果:临产后子宫体部平滑肌高表达HSP27和αB-crystallin(P<0.05)。足月未临产和临产人子宫体部平滑肌双向凝胶电泳图谱均存在4个分子量相等、等电点不同的HSP27蛋白斑点,ImageMaster 2D Platinum软件分析仅有1个HSP27蛋白斑点具有显著差异(P<0.05),余3个HSP27蛋白斑点无显著差异(P>0.05)。RT-PCR、免疫组化和免疫印迹对HSP27蛋白验证结果与蛋白质组学一致。结论:HSP27和αB-crystallin蛋白在临产后人子宫体部平滑肌高表达,提示这2个小分子热休克蛋白可能参与了临产后子宫体部平滑肌细胞的收缩事件。AIM： To investigate the expression of heat-shock protein 27 （HSP27） and ct-cystallin B chain （otB-crystallin） proteins in corpus uteri myometrium in not-in-labor and in-labor situations. METHODS： Comparative pro- teomics technique was used to identify HSP27 and otB-crystallin in corpus uteri myometfium in not-in-labor and in-labor sit- uations. The methods of haft quantitative RT-PCR, Western blotting analysis and immunohistochemistry were performed to determine the differential expression levels of HSP27. RESULTS： The protein levels of HSP27 and etB-crystallin were highly up-regulated in corpus uteri myometrium at term spontaneous labor （ P 〈 O. 05 ）. Four HSP27 spots were identified with identical molecular weight and different isoelectric points from 2 two-dimensional gel electrophoresis profiles of corpus uteri myometrium. ImageMaster 2D Platinum software analysis showed that only one HSP27 spot had differential signifi- cance （P 〈 0.05）, and the rest spots had no significant difference between the 2 profiles （ P 〉 0.05 ）. CONCLUSION ： The protein levels of HSP27 and aB-ciystallin are highly up-regulated in corpus uteri myometrium at term spontaneous la- bor, suggesting that the two small heat-shock proteins participate in human myometrial contraction at labor and will be po- tential targets for future tocolytic design.

关 键 词：子宫肌层 热休克蛋白27 α-晶体蛋白B链 子宫收缩 

分 类 号：R714.14[医药卫生—妇产科学]