流动注射化学发光法测定氢溴酸加兰他敏和力可拉敏  

Flow-injection chemiluminescence method for the determination of lycoramine and galanthamine

作　　者：宋翠翠[1] 唐玉海[1] 史喜燕[1] 张广彬[1] 李明月[1] 李昕沛[1] 

机构地区：[1]西安交通大学理学院分析科学研究所,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2012年第6期790-792,796,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的建立一种简单快速测定氢溴酸加兰他敏和力可拉敏的流动注射化学发光方法。方法在碱性条件下,加兰他敏与力可拉敏对鲁米诺-铁氰化钾发光体系有明显的抑制作用,且抑制效果与药物浓度呈线性关系。据此,建立了氢溴酸加兰他敏和力可拉敏的流动注射化学发光分析方法,并采用单因素优化方法对可能的影响分析方法的因素进行了优化。结果在优化条件下,加兰他敏和力可拉敏的线性范围分别为5×10-3～10mg/L和5×10-2～10mg/L;检出限分别为0.53μg/L和2.60μg/L。结论流动注射化学发光方法可以用于氢溴酸加兰他敏和力可拉敏药物制剂和生物样品的含量分析。Objective To develop a simple and rapid flow-injection chemiluminescence（FI-CL） method for the determination of galanthamine and lycoramine.Methods In an alkaline medium,galanthamine and lycoramine can obviously inhibit the chemiluminescence intensity of the reaction of luminol-K3Fe（CN）6 system,and the relative CL intensity is proportional to the concentration of galanthamine and lycoramine.Based on this,a new FI-CL method was developed to determine galanthamine and lycoramine.And the effects of chemical and instrumental variables were optimized.Results Under the optimized conditions,the relative CL intensity was proportional to the concentration within the range of 5×10-3-10 mg/L for galanthamine and within the range of 5×10-2-10 mg/L for lycoramine.The detection limit was 0.53 μg/L for galanthamine and 2.60 μg/L for lycoramine,respectively.Conclusion The flow-injection chemiluminescence method can be applied to determine the content of galanthamine and lycoramine in pharmaceutical preparations and in serum.

关 键 词：化学发光 鲁米诺 加兰他敏 力可拉敏 药物分析 

分 类 号：D652[政治法律—政治学]