糖皮质激素变化对高原急性缺氧后脑损伤的影响  

Effect of glucocorticoid levels on cerebral injury after high altitude hypoxic exposure

作　　者：张清花[1] 张泉龙[2] 李茂星[2] 贾正平[2] 邱建国[2] 周珺[2] 尉丽力[2] 

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]兰州军区兰州总医院药剂科全军高原环境损伤防治重点实验室

出　　处：《西北国防医学杂志》2013年第1期21-23,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：兰州军区医药卫生科研基金资助项目(CLZ12JB03)

摘　　要：目的:探讨高原急性缺氧(7000 m,24 h)下糖皮质激素变化对脑损伤和血清S100B蛋白表达的影响。方法:成年Wistar大鼠随机分为地塞米松给药组、美替拉酮给药组、高原对照组和平原对照组,第3天给药后1 h,前三组置于低压低氧模拟舱中,模拟海拔7000 m,24 h后出舱,酶联免疫法检测血清皮质酮(CORT)、S100B,干湿重法测定脑含水量及肺含水量。结果:与平原对照组比较,高原各组的CORT、S100B、脑含水量和肺含水量均明显增高(P<0.01)。与高原对照组比较,地塞米松给药组CORT显著增高(P<0.01),S100B显著降低(P<0.05),脑含水量有一定降低、肺含水量降低(P<0.05)。美替拉酮给药组CORT显著降低(P<0.01),S100B显著增高(P<0.01),脑含水量增高,肺含水量降低(P<0.05)。结论:在急性重度缺氧期,大鼠皮质酮水平增加对脑缺氧损伤有一定保护作用,其机制可能与下调S100B蛋白和稳定血脑屏障有关。Objective： To observe the effects of serum levels of glucocorticoid on cerebral injury and expression of serum S100B in rat after acute high altitude hypoxia exposure. Methods： Adult Wistar rats were randomly divided into 4 groups, which were dexamethasone pretreatment group, metyrapone pretreatment group, high altitude control group and plain control group. Following with three days pretreatment, the forward three groups were exposed to simulated 7 000 m altitude in a hypobaric chamber for 24 h, the serum CORT and S100B levels were measured by ELISA methods, and the water content of brain and lung were measured by dry - wet weight method. Results： As compared with plain control group, the levels of CORT, S100B, brain and tung water contents were significantly increased in each high altitude groups （P 〈 0.01 ）. Compared with high altitude control group, the CORT levels in dexamethasone group were increased significantly （P 〈 0.01 ） followed with decreased S100B （P 〈 0.01 ） , and brain and lung water contents （P 〈0.05） , but the CORT levels in metyrapone group were decreased and the S100B was increased significantly, and a few increase of brain water contents and decrease of lung water contents were observed （ P 〈 0.05 ）. Conclusion ： During acute high altitude hypoxia exposure, the increase of CORT can make the protective effects, which may be related with the decrease of the expression of S100B protein and the stability of blood - brain barrier.

关 键 词：高原急性缺氧 S100B蛋白 皮质酮 地塞米松 美替拉酮 

分 类 号：R651.15[医药卫生—外科学]