椒蒿对自身免疫性睾丸炎小鼠CD4+CD25+调节性T细胞的影响  

Effects of pepper artemisia on CD4+CD25+ regulatory T cells in experimental autoimmune orchitis mice

作　　者：仵燕[1] 斯依提尼沙汗.吾满尔 吴小川[1] 骆靖华[1] 玛衣拉.阿不拉克 

机构地区：[1]新疆医科大学 [2]新疆维吾尔自治区维吾尔医院药剂科,新疆乌鲁木齐830054

出　　处：《西安交通大学学报（医学版）》2013年第2期244-247,269,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30660054)~~

摘　　要：目的研究椒蒿挥发油对自身免疫性睾丸炎小鼠CD4+CD25+调节性T细胞的影响。方法雄性BALB/c小鼠80只,随机分为4组(生理盐水组、模型组、橄榄油组、椒蒿组),采用睾丸内注射冰醋酸的方法造模,各组都在手术前3天开始灌胃,手术后继续灌胃,共连续灌胃24d(盐水组只灌胃,不手术)。分别于术后3周、9周取外周血,通过流式细胞仪对CD4+CD25+调节性T细胞进行检测。结果术后3周,模型组、橄榄油组、椒蒿组小鼠外周血CD4+CD25+调节性T细胞百分比均低于生理盐水组,差异有统计学意义(P<0.05),而此3组之间差异无统计学意义(P>0.05)。术后9周模型组与橄榄油组小鼠外周血CD4+CD25+调节性T细胞百分比低于生理盐水组,差异有统计学意义(P<0.05),椒蒿组与生理盐水组比较差异无统计学意义(P>0.05)。结论椒蒿能使自身免疫性睾丸炎小鼠CD4+CD25+调节性T细胞百分比提高,后者可发挥免疫调节作用。Objective To study the effects of volatile oil from pepper artemisia leaves on CD4＋ CD25＋ regulatory T cells in murine autoimmune orchitis mice. Methods Totally 80 male BALB/c mice were randomly divided into 4 groups （normal saline group, model group, olive oil group, and pepper artemisia group）. After model making, each group received gavage three days before the surgery, and for 24 days after the surgery （saline group received only the gavage, but no operation）. CD4＋ CD25＋ regulatory T cells in the peripheral blood were detected by flow cytometry 3 and 9 weeks after the surgery. Results Three weeks after the surgery, the percentage of peripheral blood CD4＋ CD25＋ regulatory T cells in model group, olive oil group and pepper artemisia group was significantly lower than that in saline group （P〉0.05）, but the differences among the first three groups were not significant （P〈0.05）. Nine weeks after the operation, the percentage of peripheral blood CD4 ＋ CD25＋ regulatory T cells was significantly lower in model group and olive oil group than in saline group （P〈0.05）; however, pepper artemisia group and saline group did not differ significantly （P〈0.05）. Oonclusion Pepper artemisia can increase the percentage of CD4＋ CD25＋ regulatory T cells, which exert immunity-regulatory effects on autoimmune orchitis mice.

关 键 词：椒蒿 自身免疫性睾丸炎 CD4+CD25+调节性T细胞 BALB/C小鼠 

分 类 号：R392.12[医药卫生—免疫学]