帕罗西汀对大鼠海上灾害创伤后应激障碍的影响  

Experiment study of paroxetine on the therapeutical effect of posttraumatic stress disorder in rats following maritime disasters

作　　者：贺顺龙[1] 谢培增[1] 汪先兵[1] 周赤龙 朱晖 

机构地区：[1]解放军第四二一医院,广州510318 [2]解放军第四二二医院

出　　处：《海军医学杂志》2013年第1期14-17,共4页Journal of Navy Medicine

基　　金：全军医学科学技术"十一五"课题(06MB174)

摘　　要：目的探讨帕罗西汀对大鼠海上灾害创伤后应激障碍的影响。方法健康雄性sprague-dawley大鼠50只,按数字表法随机分为空白对照组(空白组不予应激和不给药)、海上应激组(应激组予以应激,不给药)、单纯用药组(单纯组不予应激,给帕罗西汀3 mg/(kg.d))、常规剂量组(常量组予以应激,帕罗西汀3 mg/(kg.d))、大剂量组(大量组予以应激,帕罗西汀10 mg/(kg.d)),每组各10只。采用海上单一延长应激(MPTSD)动物模型,即大鼠进行2 h的紧密束缚,置于医院船甲板上海水中强迫游泳20 min,乙醚麻醉至意识丧失,应用或不用帕罗西汀,常规喂养15 d。在海上创伤应激结束后第15～21天,进行旷场实验、高架十字实验、MWM水迷宫实验,分别评定大鼠对环境的恐惧反应,高警觉性,焦虑/抑郁行为以及学习记忆能力。结果在旷场实验中,常量组水平活动度为(10 817.59±1082.56)mm,中央停留时间为(50.29±5.40)s;大量组分别为(11 119.81±749.73)mm、(51.96±5.43)s,均显著高于其他组(P<0.05),大量组各参数高于常量组(P<0.05);在高架十字测试中,大鼠开臂进入次数和停留时间的百分比,常量组分别为(40.78±5.71)%、(21.98±4.38)%;大量组分别为(49.59±5.58)%、(24.67±5.50)%,均显著高于应激组(P<0.05);在MWM水迷宫实验中,上台潜伏期存在组间差异,应激组潜伏期高于空白组、常量组和大量组。应激组靶象限活动时间百分比和穿越站台次数显著低于其他组。结论早期给予帕罗西汀可以明显改善大鼠对海上环境的恐惧反应和警觉性,减轻焦虑/抑郁情绪,提高学习记忆能力。Objective To investigate the effect of paroxetine on the therapeutical effect of posttraumatic stress disorder in rats following maritime disasters. Methods Fifty sprague-dawley rats were randomly divided into 5 groups, each consisting of l0 animals ： the blank control group （animals without stress and drugs）, the maritime stress model group（ animals with stress, but without drugs）, the pure drug treatment group （animals without stress, but with paroxetine treatment, 3mg/kg, once a day）, the normal dosage group （ animals with stress and with a normal dosage of paroxetine treatment, 3 mg/kg, once a day）, the high dosage group （ animals with stress and with a high dosage of paroxetine, 10 mg/kg, once a day）. Our study used MPTSD model, i.e. 2 hours of restraint ＋ 20-minute forced swimming in seawater on the deck of the hospital ship ± anesthetization with ethyl ether till loss of consciousness. Then, the animals with or without paroxetine were raised for 15 days. At day 15 to 21, following posttraumatic stress disorder at sea, elevated plus maze （EPM）, open-field test （OF） and Morris water maze （MWM） were performed to assess rat responses to fear due to environment, high alertness, anxiety/depression behavior, and their learning and memory ability. Results In the open field test, horizontal motion distance of the animals in the normal dosage group （10 817.59 ± 1082.56） mm and central stay time was （50.29 ±5.40） s, while horizontal motion distance and central stay time of the high dosage group were （11 119.81 ± 749.73 ） mm and （51.96 ± 5.43 ） s respectively, which were significantly higher than those of other groups （P 〈 0.05）. In the elevated plus maze test, the frequency of entrance and the percentage of stay time for the normal dosage group were （40.78 ± 5.71 ） % and （21.98 ±4.38） % respectively, and those of the high dosage group were （49.59 ± 5.58 ） % and （24.67 ± 5.50） % respectively （ P 〈 0.05 ）, which were significantly

关 键 词：海上灾害 创伤后应激障碍 帕罗西汀 

分 类 号：R493[医药卫生—康复医学]