白细胞介素13对瘢痕成纤维细胞增生及转录因子c-fos表达的影响  

Effects of IL-13 on Fibroblast Proliferation and Transcription Factor c-fos Expression

作　　者：周莹[1] 熊丽霞[2] 董蕙[1] 龙飞[1] 

机构地区：[1]南昌大学基础医学院组织胚胎学教研室,南昌330006 [2]南昌大学基础医学院病理生理学教研室,南昌330006

出　　处：《南昌大学学报（医学版）》2012年第12期23-26,共4页Journal of Nanchang University：Medical Sciences

基　　金：江西省卫生厅科技计划(20112021);南昌大学校基金资助项目

摘　　要：目的研究白细胞介素13(interleukin 13,IL-13)对瘢痕成纤维细胞增生和转录因子c-fos表达的影响。方法 MTT比色法检测IL-13不同质量浓度(50、100、150、200μg.L-1)下CRL-1762细胞的增殖水平,对照组不加IL-13;RT-PCR检测100μg.L-1 IL-13在不同培养时间(15、30、60min)CRL-1762细胞表达c-fos mRNA的水平,对照组不加任何试剂;Western Blotting检测100μg.L-1 IL-13在不同培养时间(30、60、120min)对CRL-1762细胞表达c-fos蛋白质的水平,对照组不加任何试剂。结果 IL-13组CRL-1762细胞吸光度较对照组明显增强,且细胞增殖率与IL-13浓度有关;IL-13诱导CRL-1762细胞30min后,c-fos mRNA的表达较其余各组均明显增强(P<0.05);IL-13诱导CRL-1762细胞60min后,c-fos蛋白质的表达较其余各组均有显著升高(P<0.05)。结论IL-13对CRL-1762细胞的生长有明显的促进作用,并在一定范围内呈浓度依赖性;IL-13能够诱导CRL-1762细胞c-fos mRNA和蛋白质的表达瞬时增强。Objective To investigate the effects of IL-13 on fibroblast proliferation and transcription factor c-fos expression.Methods The effects of IL-13(50,100,150 and 200 μg·L-1) on the proliferation of CRL-1762 cells were determined by MTT assay.Control cells received the vehicle only.The expression of c-fos mRNA was examined by RT-PCR in CRL-1762 incubated with or without IL-13(100 μg·L-1) for 15,30 or 60 minutes.The expression of c-fos protein was detected by Western Blotting in CRL-1762 incubated with or without IL-13(100 μg·L-1) for 30,60 or 120 minutes.Results The treatment with IL-13 obviously promoted the proliferation of CRL-1762 cells in a concentration-dependent manner.After treatment with IL-13 for 30 and 60 minutes,the mRNA and protein expression of c-fos significantly increased compared with other groups,respectively(P<0.05).Conclusion IL-13 can promote the proliferation of CRL-1762 cells in a concentration-dependent manner and increase the expression of c-fos mRNA and protein.

关 键 词：白细胞介素13 CRL-1762 C-FOS 

分 类 号：Q28[生物学—细胞生物学]