CD4^+CD25^+FoxP3^+调节性T细胞消除对接触性超敏反应的影响  

Role of Depletion of CD4^+CD25^+FoxP3^+ Regulatory T Cells on Contact Hypersensitivity

作　　者：王庆辉[1] 冯永辉[1] 刘军[1] 李莹[2] 曹雅明[1] 吕昌龙[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,辽宁沈阳110001 [2]沈阳市胸科医院检验科,辽宁沈阳110044

出　　处：《微生物学杂志》2012年第6期65-68,共4页Journal of Microbiology

基　　金：国家自然科学基金(30901343)

摘　　要：接触性超敏反应(CHS)是研究体内免疫活化和调节的重要模型之一。分泌IL-17的CD4+Th(Th17)细胞亚群在CHS发展过程中发挥重要作用。CD4+CD25+FoxP3+调节性T细胞(Treg)是具有较强免疫调控作用的T细胞亚群。在氯化苦咪酸(TNCB)诱导的接触性超敏反应小鼠模型中,TNCB攻击后小鼠Treg占CD4+T细胞的百分比显著增高,于24～48 h达到峰值,72 h开始回落。进一步利用抗-CD25 mAb 7D4在小鼠体内消除Treg后,小鼠的受攻击皮肤增厚明显高于对照组。尽管CD4+T细胞在淋巴细胞中总的比例没有显著变化,但CD4+IL-17+T细胞比例显著增加。由此提示,CD4+CD25+FoxP3+Treg可能通过影响效应性Th17的功能,进而抑制CHS的发生。Contact hypersensitivity （CHS） was one of the most intensively studied in vivo models of immune activation and the regulation. IL-17 producing CD4 ＋ T helper （Th17） subset played an important role in the pathogenesis of contact hypersensitivity. CD4＋ CD25 ＋ FoxP3 ＋ regulatory T cells （Trcg） act as T subset with immunoregulatory function. In the trinitrochlorobenzene （TNCB） induced contact hypersensitivity model, the percentage of Trcg in CD4 ＊ cells significantly increased following TNCB challenge, peaked at 24 ＋48 h, and then fell at 72h. After deple- tion of Treg with injection of anti-CD25 mAb （71）4） into mice, compared with control group, ear swelling was significantly higher in Treg-depleted group. In agreement, the percentage of IL-17 producing CD4 ＋T was also enhanced followed by Trcg depletion, although the ration of CD4 ＋ T remains. These data suggested that CD4 ＋ CD25 ＋ FoxP3 ＋ Treg suppress the Clt$ possibly via influencing the function of Thl7.

关 键 词：接触性超敏反应 调节性T细胞 IL-17 

分 类 号：R392[医药卫生—免疫学]