乙型肝炎卡介苗联合疫苗免疫豚鼠及小鼠的病理学研究  

Pathology of HBV-BCG Combined Vaccine to Immunize Guinea Pigs and Mice

作　　者：蔡岩[1] 赵晓青[1] 李继宏 周捷[1] 周长军[1] 赵亚力[1] 惠琦[1] 

机构地区：[1]长春生物制品研究所有限责任公司,吉林长春130062 [2]吉林亚泰生物药业股份有限责任公司,吉林长春130033

出　　处：《微生物学杂志》2012年第6期82-86,共5页Journal of Microbiology

摘　　要：观察乙型肝炎卡介苗联合疫苗(简称HBV-BCG联合疫苗)皮下免疫豚鼠的毒性病理损伤,及其皮内免疫小鼠的局部毒性反应和可逆性改变,检查乙肝表面抗原是否为促使卡介苗毒力增强的潜在因素。豚鼠及小鼠均分设3组,即对照组、BCG组和HBV-BCG联合疫苗组。按计划免疫豚鼠及小鼠,豚鼠观察至42天全部活杀。小鼠观察至第3、5、7、9、11、15、20、25、30天各活杀3只。大体剖检,常规病理制片,HE染色,病理组织学观察和显微照相。对照组豚鼠及小鼠接种局部皮肤组织及全身各脏器均未见异常。与对照组相比,BCG组和HBV-BCG联合疫苗组豚鼠及小鼠接种局部皮肤均发生非特异性炎症反应,以及由BCG引起的特异性类上皮细胞肉芽肿性炎。2组接种局部皮肤的病理改变相似,未见明显差异。另外豚鼠蹊部、肺门和肠系膜淋巴结皮质内的淋巴滤泡有不同程度增生,生发中心活跃、扩大,副皮质区增宽。肺、肝、脾及其余各脏器均未见与疫苗相关的病理改变。BCG或HBV-BCG联合疫苗免疫豚鼠及小鼠所产生的病变,具有BCG原发性病变特征,均未见干酪样坏死或转向恶性化的结核性病变。证实HBsAg不具有促使BCG毒力增强的潜在危险。Hepatitis BCG combined vaccine （HBV-BCG vaccine ） subcutaneous immunization on guinea pigs with toxic pathological injury and intradermal immunization on mice with the local toxic reaction and reversible change were observed in order to inspect whether HBsAg was the potential factor to promote BCG ＇ s toxicity. The guinea pigs and mice were evenly divided into three groups, i.e. control group, BCG group, and HBV-BCG combined vaccine group. The guinea pigs and mice were immunized according to the plan, and the guinea pigs were entirely killed alive by 42nd days＇ observation, and by 3rd, 5th, 7th, 9th, llth, 15th, 20th, 25th, 30th days＇ observation the mice were respectively killed 3 each, and carried out general necropsy, routine pathologic slide making, HE staining, histopathological observation and microscopic photography. The results showed that the control group of guinea pigs and mice inoculated with local skin tissues and the whole organs were not seen abnormal. As compared with the control group, local skin on the inoculated sites in guinea pigs and mice of BCG group and HBV-BCG combined vaccine groups emerged nonspecific inflammatory reaction and the specificity of epithelioid cell granuloma inflammation caused by BCG. The local skin pathological changes on the inoculated sites of the two groups were similar, no significant difference were seen. In addition, lymphoid follicles of the guinea pig＇s foot, hi]us pulmonis, mesenterie lymph node cortex, there were different degree of hyperplasia, active and expanded germinal center, paracortical zone broadening were observed. No vaccine associated pathological changes on lung, liver, spleen and the rest organs were seen. Therefore, pathological changes emerged with the immunization with BCG or HBV-BCG combined vaccine on the guinea pigs and mice possessed the pathological characteristics of the primary pathological change characteristics, no caseous necrosis or malignant-turning tuberculous pathology were seen. Thus it was confirmed that the HBsAg di

关 键 词：乙型肝炎 卡介苗 联合疫苗 病理学 

分 类 号：R373.21[医药卫生—病原生物学]