卡介苗干预对哮喘小鼠肺组织IL-17A表达的影响及可能机制研究  

Effect and Possible Mechanisms of the BCG on IL-17A Expression in the Lung of Asthmatic Mice

作　　者：陈红梅[1] 黄信刚[2] 曾晓媛[1] 陈华妹[2] 唐炳松[2] 

机构地区：[1]长沙市中心医院胸科中心,湖南长沙410007 [2]中南大学湘雅二医院呼吸内科,湖南长沙410007

出　　处：《现代生物医学进展》2012年第35期6824-6826,6861,共4页Progress in Modern Biomedicine

摘　　要：目的:观察卡介苗干预对哮喘小鼠肺组织IL-17A的表达、气道炎症的影响,并探讨可能机制。方法:按随机数字表法,24只昆明小鼠分为正常对照(A组),模型组(B组),卡介苗(BCG)干预组(C组)。C组小鼠每周一次皮内注射BCG 0.025 mg,连续3次。首次皮内注射4周后,第1、8、15天每只小鼠分别给予鸡卵清蛋白(OVA)与氢氧化铝混合腹腔注射,第22天给予1%OVA溶液雾化吸入激发。激发后收集支气管肺泡灌洗液(BALF)行细胞总数及分类计数。肺组织HE染色行支气管粘膜下炎症评分。酶联免疫吸附试验法(ELISA)检测肺组织匀浆中的IL-17A和IFN-γ水平。结果:与正常对照组比较,BCG干预小鼠BALF中白细胞总数及中性粒细胞、嗜酸性粒细胞、淋巴细胞、巨噬细胞数均高于显著增高,低于模型组小鼠。BCG处理小鼠支气管粘膜下炎性细胞浸润程度较模型组小鼠明显降低(P<0.01),比A组明显(P<0.01)。与对照组相比,模型组和BCG组小鼠肺组织匀浆中的IFN-γ显著相抵,在模型组小鼠更明显,IL-17A浓度增高,在模型组小鼠更明显(P<0.05)。结论:BCG干预可抑制气道炎症,可能通过上调Th1型免疫反应增加IFN-γ浓度、减少IL-17A在哮喘小鼠肺组织中表达,减少中性粒细胞的募集活化。Objective： To explore the effect of BCG on the expression of IL-17A and airway inflammation iof lung in a asthmatic mice.Methods： 24 Kunming mice were randomly divided into control（A）,model group（B） and BCG group（C）.Rats in BCG group were given intradermal injection BCG 0.025 mg three consecutive times,weekly.4 weeks after the firsrt intrademal injection,rats in ECG group were exposed to intraperitoneal injection of mixture of OVA and aluminum hydroxide at day1,day 8 and 15.1% OVA inhalation solution to stimulate was administered at day 22.Total cell counting and cell differentiating in bronchoalbveolar lavage fluid（BALF） were performed.A semi-quantified method was used to evluate airway inflammation after hematoxylin-eosin（HE） staining.ELISA assay（ELISA） was performed for detection of IL-17A and IFN-γexpression in lung tissue.Results： When compared with control,the cell counting in BALF,neutrophils,eosinpphils,lymphocytes,leukocidins in BALF of BCG rats increased obviously,but decreased in model rats.More inflammatory cell infiltration could be found in tunica mucosa bronchiorum from model rats and BCG rats,while more obviously in model rats,with significant difference P0.01.Increased IL-17A and decreased IFN-γ concentration could be found in model rats and BCG rats and much more obviously in model rats,with significant difference（P0.05）.Conclusions： BCG injection could reduce the airway inflammation,probably due to increase Th1-type immune response and reduce IL-17A in the lung tissues of asthmatic mice.

关 键 词：哮喘 卡介苗 IL-17A IFN-Γ 气道炎症 

分 类 号：Q953[生物学—动物学] R562.25[医药卫生—呼吸系统]