散射比浊法检测糖化血红蛋白及临床意义  

Clinical Significance of Scattering Turbidimetric Method for Glycosylated Hemoglobin Detection

作　　者：喻飞 王佑清 周本霞 刘俊[2] 毛大勇[3] 霍治[4] 

机构地区：[1]郧西县人民医院检验科,湖北郧西442600 [2]武钢二医院,湖北武汉430000 [3]湖北医药学院附属太和医院,湖北武汉430000 [4]中南大学湘雅医学院,湖南长沙410013

出　　处：《现代生物医学进展》2012年第35期6846-6848,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31200141)

摘　　要：目的:探讨散射比浊法检测糖化血红蛋白的临床应用价值;方法:采用散射比浊法检测糖化血红蛋白,分析其检测的精密度、线性关系、抗干扰能力及相关性分析。结果:散射比浊法批内CV分别为3.1%和4.3%,批间CV分别为3.8%和5.0%;线性关系Y=1.025X+0.024,R2=0.996;胆红素、三酰甘油、尿素抗干扰实验实测值均在可信区间;散射比浊法与色谱法相关性方程为Y=0.968X+0.364,R=0.976,(P>0.05)。结论:散射比浊检测糖化血红蛋白精密度较高,线性关系良好,抗干扰能力强,相关性良好,可适用于基础和临床实验。Objective： To explore the clinical application value of scattering turbidimetric method in detecting glycosylated hemoglobin;Method： Scattering turbidimetric method was used to detect glycosylated hemoglobin,analyzes its detection precision,linear relations,the anti-interference ability and correlation.Results： Scattering turbidimetric intra-batch CV were 3.1% and 4.3% respectively,inter-batch CV were 3.8% and 5.0% respectively;Linear relationship Y = 1.025 X ＋ 0.024,R2 = 0.996;in anti-interference experiment,the measure value of Bilirubin,triacylglycerol and urea were in the confidence interval.The correlation equation for scattering turbidimetric method and chromatography is Y = 0.968 X ＋ 0.364,R = 0.976,（P 0.05）.Conclusion： Scattering turbidimetric method for glycosylated hemoglobin detection had a higher degree of precision,good linear relationship,strong anti-jamming ability,and good correlation,so it can be used in basic and clinical experiments.

关 键 词：糖化血红蛋白 散射比浊法 精密度 线性关系 抗干扰 相关性 

分 类 号：R446[医药卫生—诊断学]