再障患者血清IL-2、TNF-α、IFN-γ和外周血T淋巴细胞亚群变化研究  

Research on the Change of Aplastic Anemia Patients Serum IL-2,TNF-α,IFN-γ and Peripheral Blood T Lymphocyte Subsets

作　　者：王成红[1] 姚晨姣[1] 唐雪元[1] 范自力[1] 王二华[1] 

机构地区：[1]中南大学湘雅三医院血液科,湖南长沙410013

出　　处：《现代生物医学进展》2012年第36期7077-7079,共3页Progress in Modern Biomedicine

摘　　要：目的:研究再生障碍性贫血(AA)患者患病过程中血清IL-2、IL-8、TNF-α、IFN-γ和外周血T淋巴细胞亚群变化与疾病严重程度相关性。方法:选取我院经酶联免疫吸附法(ELISA)筛选的AA患者40例,其中急性患者(AAA)20例,慢性患者(CAA)20例,随机抽取20例健康人,对比监测60例目标病例的血清IL-2、IL-8、TNF-α、IFN-γ和外周血T淋巴细胞亚群变化。结果:AA组患者中AAA的各项细胞因子CD3+、CD4+、CD4+/CD8+检测结果较CAA患者波动较大。AA患者的血清IL-2、IL-8、TNF-α、IFN-γ含量明显高于健康人员平均值。AA患者的CD4+、CD4+/CD8+低于含量明显低于健康人员平均值,CD3+变化差异无统计学意义,AA组CD8+显著高于对照组,差异有统计学意义,P<0.05。结论:再障患者发病过程中同时存在细胞免疫功能障碍和体液免疫机制异常两种情况,且变化规律与疾病轻重缓急程度正相关。Objective：To study the correlation between changes of serum IL-2,IL-8,TNF-α,IFN-g and peripheral blood T lymphocyte subsets of aplastic anemia（AA） patients and disease severity.Methods：40 AA patients were selected by enzyme linked immunosorbent method（ELISA） screening in our hospital,including 20 acute patients（AAA） and 20 chronic（CAA） patients.Another 20 healthy people were randomly selected.Changes of serum IL-2,IL-8,TNF-α,IFN-g and peripheral blood T lymphocyte subsets of the 60 cases were monitored and compared.Results：In the AA group,detection results of each cell factor CD3＋,CD4＋,CD4＋/CD8＋ of AAA patients fluctuated greater than that of CAA patients.The serum levels of IL-2,IL-8,TNF-α,IFN-g of AA patients was obviously higher than the average levels of health persons.The levels of CD4＋,CD4＋ /CD8＋ of AA patients were significantly lower than the average levels of health persons.But the changes of CD3＋ the difference had no statistical significance.CD8＋ levels of AA patients were significantly higher than those in the control group,with was statistical difference,P0.05.Conclusion：Patients with aplastic anemia had cellular immune function obstacle and humoral immunity mechanism anomaly as well,and the changing regulations were positively correlated with the severity and acute degree of disease.

关 键 词：贫血 再生障碍性 白细胞介素 肿瘤坏死因子 

分 类 号：R556.5[医药卫生—血液循环系统疾病]