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作 者:管莹[1] 高茜[1] 朱洲海 米其利[1] 李雪梅[1] 缪明明[1] 夭建华[1]
出 处:《化学与生物工程》2013年第2期64-66,共3页Chemistry & Bioengineering
基 金:云南省烟草化学创新团队建设项目(2009CI014);云南省应用基础研究项目(2009ZC190M);云南省中烟工业公司项目(2011JC02)
摘 要:为了提高体外微核试验结果的可重复性和可比性,通过依据不同判定标准对同一受试物诱发细胞微核率进行统计、检测同一受试物对不同代次CHO细胞的微核率,研究了判定标准、细胞连续传代代次对体外微核试验结果的影响。结果表明,判定标准的不同对同一受试物诱发细胞微核率有显著影响;随细胞连续传代代次的升高,同一受试物对CHO细胞微核率呈上升趋势,连续传代9代以内细胞微核率较为稳定。判定标准、细胞连续传代代次是体外微核试验需要控制的重要因素。In order to improve the repeatability and comparability of in vitro micronueleus test, the effects of scoring criteria and cell passage on micronucleus test had been explored by counting the micronucleus rate of CHO cells induced by the same test matter according to different scoring criteria,and testing the micronucleus rate of different passages CHO cells evoked by the same test matter. The results showed that scoring criterion had siginificant effect on the micronucleus rate and the micronucleus rate induced by the same test matter in- creased with cell passage. The micronucleus rate was reliable within passage of 9. Scoring criterion and cell pas- sage were the important factors for quality control of in vitro micronucleus test.
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