正交优选超声波辅助提取三明药用植物金线莲中总氨基酸的工艺研究  

Optimization of Ultrasonic-Assisted Extraction Process of Total Amino Acids from Sanming Medicinal Plant Anoectochilus Roxburghii by Orthogonal Experiment

作　　者：张茶妹[1] 周文富[1] 

机构地区：[1]三明学院化学与生物工程系,福建三明365004

出　　处：《化学与生物工程》2013年第2期78-82,共5页Chemistry & Bioengineering

摘　　要：研究了三明药用植物金线莲中总氨基酸的超声波辅助提取工艺。在单因素实验的基础上,通过L9(34)正交实验确定最佳提取条件为:乙醇体积分数60%、料液比1∶40(g∶mL)、超声波功率60W、提取温度60℃、提取2次、每次提取时间40min,在此条件下,总氨基酸的提取率达9.86%。该法具有简单、快速、高效、无需加热、提取率高等优点。The ultrasonic-assisted extraction process of total amino acids from Sanming medicinal plant Anoectochilus roxburghii was investigated. Based on single factor experiment, the optimum extraction condi- tions were obtained by orthogonal experiment as follows：ethanol volume fraction of 60%,solid-liquid ratio of 1 ： 40（g · mL） ,ultrasonic power of 60 W,extraction temperature of 60 ℃ ,ultrasonic extraction of 9 times,each for 40 min. Under above conditions,the extraction rate of total amino acids was 9.86%. The method is simple, fast, efficient, without heating, and with high extraction rate.

关 键 词：金线莲 总氨基酸 超声波辅助提取 HPLC 

分 类 号：TQ028[化学工程] R284.1[医药卫生—中药学]