新藤黄酸对黑色素瘤B16细胞凋亡的作用  

Effect of Gambogenic Acid on Apoptosis of Melanoma Cell Line B16

作　　者：张璇[1] 王梅[1] 程卉[1] 苏婧婧[1] 李庆林[1] 

机构地区：[1]省部共建新安医学教育部重点实验室安徽中医学院科研实验中心,安徽合肥230038

出　　处：《安徽中医学院学报》2013年第1期53-56,共4页Journal of Anhui Traditional Chinese Medical College

基　　金：国家自然科学基金项目(81173600);安徽省自然科学基金项目(11040606M190);国家科技重大专项-新药创制项目(2009ZX09103-399)

摘　　要：目的探讨新藤黄酸(gambogenic acid,GNA)对小鼠黑色素瘤B16细胞凋亡的影响。方法用不同浓度的GNA培养B16细胞24h,倒置显微镜下观察细胞形态;采用四甲基偶氮唑蓝(methyl thiazolyl tet-razolium,MTT)法测定GNA对B16细胞增殖的抑制作用;荧光显微镜观察吖啶橙/溴化乙锭(acridine or-ange/ethidium bromide,AO/EB)染色后细胞凋亡;采用膜联蛋白Ⅴ-异硫氰酸荧光素/碘化丙啶双染,流式细胞仪检测B16细胞凋亡。结果 GNA作用B16细胞后,细胞的抑制率随着药物浓度的增加而升高;AO/EB染色荧光显微镜观察显示GNA处理的B16细胞出现了典型的凋亡特征;流式细胞仪检测显示GNA处理的B16细胞随着药物浓度的增大凋亡率随之上升。结论 GNA在一定的范围内,能够浓度和时间依赖性地通过诱导细胞凋亡抑制小鼠黑色素瘤B16细胞的增殖。Objective To investigate the effect of gambogenic acid （GNA） on the apoptosis of mouse mela- noma cell line B16. Methods B16 cells were cultured with different concentrations of GNA for 24 h. The morphology of B16 cells was observed under an inverted microscope; the inhibitory effect of GNA on the proliferation of B16 cells was evaluated by methyl thiazolyl tetrazolium assay; the apoptosis of B16 cells was observed by acridine orange （AO）/ethidium bromide （EB） staining under a fluorescence microscope; the apoptosis rate of B16 cells was measured by annexin V-fluorescein isothiocyanate （FITC）/propidium i- odide （PI） double staining and flow cytometry. Results After treatment with GNA, the inhibition rate of B16 cells increased as the concentration of GNA rose; the B16 cells stained with AO/EB showed typical morphological changes of apoptosis under the fluorescence microscope; the apoptosis rate of B16 cells in- creased as the concentration of GNA rose, as measured by flow cy~;ometry. Conclusion GNA can inhibit the proliferation of B16 cells through inducing cell apoptosis, and the inhibitory effect is in a time-depend- ent and concentration-dependent manner within a certain range of GNA concentrations.

关 键 词：新藤黄酸 B16细胞 细胞增殖 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]