补肾解毒方与健脾解毒方含药血浆对慢性乙肝病毒感染不同免疫状态患者外周血T细胞功能的影响  

Effect of tonify kidney and detoxification as well as strengthening spleen and detoxification decoctions on T cells in patients with hepatitis B virus infection in different immune status

作　　者：欧松[1,2] 孙克伟[1] 彭建平[1] 祁双林[1] 闻杰[1] 胡莉[1] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]衡阳市第三人民医院

出　　处：《中西医结合肝病杂志》2013年第1期29-32,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家自然科学基金资助项目(No.81072779);教育部博士学科点科研基金项目(No.20114323110002);湖南省自然科学基金项目(No.09JJ6042)

摘　　要：目的:比较补肾解毒方和健脾解毒方对慢性乙肝病毒(HBV)感染不同免疫状态患者外周血T淋巴细胞(Ts)功能的影响。方法:36例门诊慢性HBV感染患者,根据患者免疫状态分为免疫耐受组(18例)、免疫清除组(18例),另选择10名健康人作健康组。分别采集抗凝外周静脉血,分离外周血单个核细胞(PBMCs),进一步分离提取培养T细胞,采用大鼠补肾解毒和健脾解毒药物血浆进行干预。检测T细胞亚群CD3+、CD4+、CD8+以及CD28+分子的表达,观察T细胞培养上清液干扰素-γ(IFN-γ)的水平。结果:健脾解毒药物血浆与补肾解毒药物血浆干预后CD3+、CD4+、CD8+以及CD28+分子表达率升高,且补肾解毒药物血浆提高CD28+表达率优于健脾解毒药物血浆(P<0.05)。两种含药血浆干预后T细胞分泌的IFN-γ水平较干预前升高,差异有显著性意义(P<0.05)。结论:补肾解毒方和健脾解毒方都能促进慢性HBV感染患者T细胞功能的恢复,且两种含药血浆对慢性HBV感染免疫耐受期患者T细胞功能的影响更为显著。Objective： To investigate the effect of TCM decoction （ Bushenjiedu Tang and Jianpijiedu Tang） on T cells in patients with chronic hepatitis B virus infection in different immune status. Methods： Thirty-six out-patients with chronic HBV infection were divided into immune tolerance group （ 18 cases ）, immune clearance group （ 18 cases） according to their im- mune status, and the other 10 healthy persons were selected as control group. Ts （T cells） subset populations were diferentiated from peripheral blood mononuclear cells （PBMCs） , incubated and induced then, the Ts were interfered by plasma of medicine of Bushenjiedu decoction and Jianpijiedu decoction, respectively. The expressions of CD3 ^＋ , CD4 ^＋ , CD8 ^＋ and CD28 ＊ were detected by FACS, and the levels of IFN-~/in supernatants by ELISA. Results： After the drugs intervention , the CD3 ^＋ , CD4 ^＋ , CD8 ^＋ and CD28 ＋ expression levels of immune tolerance group and the immune clearance group were higher than the control （health） group, and the CD28 ＋ expression levels of tonify kidney and detoxification group were higher than the immune clear- ance group , the difference was statistically significant （P 〈 0. 05） . After the drugs intervention , the IFN-γexpression levels of immune tolerance group and the immune clearance group were higher than before the intervention, and the difference was statisti- cally significant （P 〈 0. 05 ） . Conclusion： The tonify kidney and detoxification moderate doses side and strengthening spleen and detoxification small doses side can promote Ts out patients with chronic hepatitis B virus infection in state of immune tolerance and immune clearance. The influence of tonifying kidney and detoxification and strengthening spleen and detoxification on Ts function in chronical HBV infectious patients in immune tolerance stage was much more prominent.

关 键 词：补肾解毒方 健脾解毒方 乙型肝炎病毒 外周血 T细胞 

分 类 号：R259[医药卫生—中西医结合]