地衣芽孢杆菌YB15发酵培养优化的研究  

A Study of Optimal Fermentation Culture of Bacillus lincheniformis YB15

作　　者：朱天辉[1] 李姝江[1] 向潇潇[1] 谯天敏[1] 

机构地区：[1]四川农业大学林学院,四川雅安625014

出　　处：《四川林业科技》2013年第1期1-4,共4页Journal of Sichuan Forestry Science and Technology

基　　金：国家自然科技资源共享平台(2005DKA21207-13)

摘　　要：采用单因素试验和正交试验对地衣芽孢杆菌(Bacillus lincheniformis)YB15发酵培养基的碳源、氮源、无机盐进行研究,得出该菌的最适培养基组分为葡萄糖5 g,酵母浸膏7.5 g,蛋白胨7.5 g,硫酸铵5.0 g。在此基础上运用正交试验对地衣芽孢杆菌YB15发酵的最适条件进行研究,得到的最适培养条件为温度25℃,pH 6.5,瓶装量100 mL,接种量4 mL;地衣芽孢杆菌生长曲线的结果表明12 h～18 h时为菌体收集的最佳时间。In this paper, by adopting the single-factor and orthogonal experiments the researches were con- ducted on the fermentation medium of Bacillus licheniformis YB15, and the results showed that its opti- mum components were glucose 5.0 g,yeast extract 7.5 g, peptone 7.5 g and ammonium sulphate 5.0 g. On this basis, its optimum fermentation conditions were also studied, which were temperature at 25 ℃, pH 6. 5 ,culture broth quantity 100 mL . 300 mL-1 flask and inoculation quantity of 4 mL. The result of the growth curve of Bacillus licheniformis showed that 12 - 18 hours were the best time to collect it.

关 键 词：地衣芽孢杆菌 发酵培养基 组分优化 生长曲线 

分 类 号：S769[农业科学—森林保护学]