天芪平颤颗粒对帕金森病大鼠异动症行为学及信号转导蛋白表达的影响  

Effects of “Tianqi Pingchan Gradule” on Dyskinesia Behavior and Expression of Signal Transduction Protein in Rats with Parkinson's Disease

作　　者：吴娜[1] 宋璐[1] 杨新新[1] 魏江磊[2] 刘振国[1] 

机构地区：[1]上海交通大学医学院附属新华医院神经内科,上海200092 [2]上海中医药大学附属曙光医院,上海200032

出　　处：《上海中医药大学学报》2013年第1期55-59,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81071025;81171203);上海市科委基础研究重点项目(09JC1411000);上海市教委科研创新重点项目(10ZZ72)

摘　　要：目的:研究天芪平颤颗粒对帕金森病(PD)大鼠异动症行为学及信号转导蛋白DARPP-32、ERK磷酸化表达的影响。方法:通过6-羟基多巴(6-OHDA)立体定向至大鼠前脑内侧束建立帕金森病模型,共25只,随机分为5组,每组5只。PD组:腹腔注射0.2%维生素C液;西药组:腹腔注射左旋多巴甲酯29 d;中药小剂量组、中剂量组、大剂量组:给予左旋多巴甲酯基础上分别加用不同剂量天芪平颤颗粒;另设假手术组为对照组(n=5)。评估不同剂量天芪平颤颗粒对PD模型大鼠运动并发症的行为学影响,并用免疫组织化学方法检测大鼠纹状体区磷酸化的DARPP-32和ERK表达情况。结果:天芪平颤颗粒能减少左旋多巴制剂产生的剂峰旋转次数。免疫组化显示,与PD组大鼠比较,西药组磷酸化DARPP-32(Thr75)明显降低,中剂量组和大剂量组均未出现明显的降低(P>0.05)。与PD组磷酸化ERK1/2比较,左旋多巴长期治疗后,磷酸化ERK1/2表达升高,中剂量组和大剂量组表达量均未出现明显上升(P>0.05)。结论:天芪平颤颗粒可以减少PD大鼠剂峰异动行为,可能通过DARPP-32(Thr75)及ERK1/2磷酸化,达到调节胞内异常信号转导的功能。Objective： To study the effects of “Tianqi Pingchan Gradule” on the dyskinesia behavior and the expressions of DARPP-32 and ERK phosphorylations in rats with Parkenson＇ s disease （PD）. Methods： The parkinsonian model was established by injecting 6-OHDA to right medial forebrain bundle （ MFB）, and the model rats were randomly divided into five groups with five rats in each group. The PD group was treated by intraperitoneal injection with 0.2% vitamin C, the western medicine group was treated by intraperitoneal injection with levodopa methyl ester for 29 days, the Chinese medicine groups （low, medium and high doses ） were treated by intraperitoneal injection with levodopa methyl ester plus “ Tianqi Pingchan Gradule” intragastrically, and another five rats were served as control group （sham-operation group）. The dyskinesia behavior of PD model rats was evaluated, and the expressions of DARPP-32 and ERK phosphorylations were detected by immunohistochemical method. Results： “Tianqi Pingchan Gradule” can reduce the peak rotation caused by long-time administration of levodopa methyl ester. The immunohistochemical results showed ： compared with the PD group, the expression of phosphorylation DARPP-32 significantly decreased in western medicine group, and there was no obvious decrease in Chinese medicine groups （ medium and high dose ） （ P 〉 0.05 ） ; compared with the PD group, the expression of phosphorylation ERK1/2 significantly increased in western medicine group, and there was no obvious increase in Chinese medicine groups （ medium and high dose） （ P 〉 0.05 ）. Conclusion ： “Tianqi Pingchan Gradule” can decrease the dyskinesia behavior of PD rats, and its mechanisms may have relationship with reversing the phosphorylations of DARPP-32 and ERK1/2, and regulate the abnormal signal transduction.

关 键 词：帕金森病 异动症 天芪平颤颗粒 ERK DARPP-32 大鼠 

分 类 号：R285.5[医药卫生—中药学]