益母草水苏碱抑制去甲肾上腺素诱导心肌细胞胚胎基因再表达的作用  

Inhibitory effects of Stachydrine of Leonurus on cardiaomyocyte hypertrophy induced by norepinephrine

作　　者：单晓莉[1] 章忱[1] 廖月玲[1] 卫洪昌[1] 吕嵘[1] 

机构地区：[1]上海中医药大学基础医学院病理教研室,上海201203

出　　处：《上海中医药杂志》2013年第2期70-72,共3页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30973821);上海市教委重点学科项目(J50301);上海市教委科研创新项目(10YZ52);上海市教委预算内科研项目(2010JW 01)

摘　　要：目的观察益母草水苏碱(stachydrine,Sta)对去甲肾上腺素(norepinephrine,NE)诱导的新生大鼠心肌细胞胚胎基因再表达的抑制作用。方法采用II型胶原酶分离新生大鼠心肌细胞,随机分为空白对照组、NE组、卡维地洛组(Car组)及益母草水苏碱组(Sta组)各组分别予相应的干预措施。采用荧光标记F-actin染色法测定心肌细胞表面积,Protein/DNA比值代表细胞蛋白合成,并以荧光定量PCR检测胚胎基因α-MHC、β-MHC mRNA表达情况。结果与空白对照组比较,NE组心肌细胞表面积、Protein/DNA比值以及β-MHC mRNA表达差异有统计学意义(P<0.05)。与NE组比较,Sta组及卡维地洛组心肌细胞表面积、Protein/DNA及β-MHCmRNA表达差异有统计学意义(P<0.05)。结论 Sta能够显著抑制NE诱导的心肌细胞肥大。Objective To observe the effects of Stachydrine of Leonurus on cardiac myocyte hypertrophy induced by norepinephrine. Methods Neonatal rat ventrieular cardiomycytes were isolated from 1-2 day-old-rat by collagenase type II enzyme. Cardiac myocytes were then treated with norepinephrine （ 1 μmol/L） for 72h in the presence or absence of earveditol （ as a positive control, 1μmol/L）, or stachydrine （ 10 μmol/L）, while controls left in medium without treatment. For cell surface area measurement, cardiac myocytes were labeled with Alexa Fluor 488 conjugated phalloidin and observed by a fluorescent microscopy. Protein synthesis was evaluated by the ratio of Protein/DNA. Where Protein concentration was determined by BCA protein assay; DNA concentration was determined with Hoescht 33258 by calf thymus DNA as a standard. The mRNA expression of two isoforms of myosin heavy chain （MHC）, α-MHC and β-MHC were measured by Real-time PCR. Results NE significantly increased the cell area, protein content, Protein/DNA ratio, β-MHC expression and β-MHC ratio in cultured cardiomyocyte （ P 〈0. 05 ）. While these changes were significantly abrogated by stachydrine （ P 〈 0.05 ）. Conclusion Staehyddne attenuate NE-induced cardiomyocytes hypertrophy.

关 键 词：益母草水苏碱 胚胎基因 心肌细胞 去甲肾上腺素 

分 类 号：R285.5[医药卫生—中药学]