2009-2011年杭州地区儿童人类偏肺病毒感染状况及病原特征分析  

Analysis of infection status and pathogenic features of human metapneumovirus among children in Hangzhou between year 2009 and 2011

作　　者：寇宇[1] 于新芬[1] 潘劲草[1] 李钧[1] 文艳苹[1] 周银燕[1] 

机构地区：[1]杭州市疾病预防控制中心微生物检验科,310021

出　　处：《中华预防医学杂志》2013年第1期31-34,共4页Chinese Journal of Preventive Medicine

基　　金：杭州市科技局资助项目（20110833836,20120533Q24）

摘　　要：目的了解杭州地区儿童急性呼吸道感染病例中人类偏肺病毒（hMPV）感染状况及病原特征。方法选取2009年11月至2010年1月、2010年11月至2011年1月杭州3家医院儿科门诊、重症监护室14岁以下急性呼吸道感染患儿372例作为研究对象，采集其咽拭子351份、鼻咽吸取物9份、气管吸取物8份、痰液4份，共372份标本。提取总核酸，用RT—PCR扩增hMPV的核蛋白（N）基因片段，取扩增阳性产物进行基因测序和序列分析，扩增阳性的标本用非洲绿猴肾细胞（Vero—E6）培养hMPV，同时，用荧光定量RT-PCR方法检测呼吸道其他病毒共感染情况。结果372份患儿标本检测hMPV的N基因阳性42份，检出率为11．2％。男性患儿hMPV阳性率为11．5％（26／226），女性患儿为10．9％（16／146），差异无统计学意义（X。=0．026，P〉0．05）。感染患儿年龄最小2个月，最大14岁，发病年龄中位数为24个月。取15份RT—PCR扩增阳性产物进行基因序列分析，结果均为B1亚型，与广东株GD165相似度为98．1％～99．5％，与北京株BJ1897相似度为87．8％～89．2％。hMPV与其他呼吸道病毒共同感染率为45．2％（19／42），其中与呼吸道合胞病毒共同感染检出率最高，为26．1％（11／42）。结论杭州地区部分儿童的急性呼吸道感染与hMPV有关，hMPV为单一基因型，与其他呼吸道病毒存在共同感染的现象。Objective To study the infection status and pathogenic features of human metapneumovirus （hMPV） among children with acute respiratory tract infection in Hangzhou. Methods A total of 372 children less than 14 years old with acute respiratory tract infectious were recruited as subjects from the pediatric clinic or intensive care unit （ICU） of 3 hospitals in Hangzhou during November 2009 to January 2010, and November 2010 to January 2011. A total of 372 specimens were collected, including 351 respiratory swab,9 nasopharyngeal aspirate material,8 endotracheal aspirate material and 4 sputum. The total nucleic acid was then extracted from the specimens, and the nucleoprotein （N） gene of hMPV was amplified by RT-PCR, whose positive products were sequenced and analyzed. Africa green monkey kidney cells （Veto-E6） were applied to culture hMPV among the positive samples; meanwhile fluorescence quantitative RT-PCR was adopted to test other respiratory virus infection. Results ： Out of 372 patients,42 （ 11.2% ） were positive for N gene of hMPV. The positive rate of hMPV among boys was tl. 5% （26/226）, and correspondingly 10.9% （16/146） among girls. The difference showed no statistical significance （X2 = 0. 026, P 〉 0.05 ）. The youngest patient was only 2 month-old and the eldest patient was 14 years old. The median of the patients＇ age was 24 months. Fifteen positive samples amplified by RT-PCR were sequenced, and all turned out to be subtype B1 ; whose similarity to GD165 found in Guangdong was 98. 1% - 99. 5% and similarity to BJ1897 in Beijing was 87.8% -89. 2%. The co-infection rate between hMPV and other respiratory virus was 45.2% （19/42） ; most of which was between hMPV and respiratory syncytial virus, whose rate at 26. 1% （11/42）. Conclusion hMPV was the single genotype relevant with the acute respiratory tract infection disease among children in Hangzhou district; however, the co-infection with other respiratory virus did exist.

关 键 词：儿童 呼吸道感染 变性肺病毒 基因 

分 类 号：R72[医药卫生—儿科]