磁珠富集法随机筛选大菱鲆基因组SNP标记  

Random SNPs Discovery from Genome and Target Gene of Turbot(Scophthalmus maximus) by Using Magnetic Beads

作　　者：董晓丽[1,2] 徐建勇[1] 陈松林[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070

出　　处：《水产学杂志》2013年第1期1-4,共4页Chinese Journal of Fisheries

基　　金：国家自然科学基金项目(31072202);863项目(2012AA092203)资助

摘　　要：利用磁珠富集法随机筛选了大菱鲆(Scophthalmus maximus)基因组7820bp的序列,获得了35个SNP标记,SNP标记的含量约为0.448%。超过68%的SNP标记由碱基转换造成,不足29%的SNP标记由碱基的颠换造成。使用磁珠富集法对目的基因KC70的检测发现,725bp的片段上发现7个SNP标记。此结果证实,该方法不仅能够随机筛选基因组SNP标记,还能筛选目的基因的SNP标记。The Magnetic Beads was used for randomly discovering SNPs from turbot（Scophthalmus maximus） genome DNA.35 SNPs were detected from 7820 bp DNA sequences,about 0.448%.More than 68 percents of the SNPs were caused by base transition and less than 29 percents resulted from transversion.Seven SNPs were found from the 725 bp fragment of KC70 gene.The findings suggest that the new method be not only useful for random SNPs discovery but also for the SNPs discovery of target genes.

关 键 词：SNP CELI BST 大菱鲆 随机 

分 类 号：S917.4[农业科学—水产科学]