糖尿病肾损害大鼠血清抗血管紧张素Ⅱ1型受体自身抗体水平变化  

Changes of Autoantibodies Against the Angiotensin Ⅱ Type 1 Receptor in Diabetic Renal Damage Rats

作　　者：白伟伟[1] 赵林双[1] 刘晔[1] 谭学莹[1] 

机构地区：[1]广州军区武汉总医院内分泌科,武汉430070

出　　处：《微循环学杂志》2013年第1期16-17,20,I0001,共4页Chinese Journal of Microcirculation

基　　金：湖北省卫生厅资助项目(JX5C18)

摘　　要：目的:观察糖尿病(DM)大鼠血清抗血管紧张素Ⅱ1型受体自身抗体(AT1-AA)与其肾损害的关系。方法:Wistar雄性大鼠36只,其中29只采用腹腔注射链脲左菌素(STZ)法成功复制DM模型(DM组),另7只用作对照(N组)。16周后,应用ELISA检测两组大鼠血清AT1-AA水平,根据检测结果将DM组AT1-AA阳性大鼠定为A组,AT1-AA阴性大鼠定为B组,测定各组大鼠血糖、24h尿白蛋白排泄率(UPE)、血尿素氮(BUN)、血肌酐(Scr)水平。结果:DM组大鼠中分布于A组11只(37.93%),分布于B组18只(62.07%),N组大鼠中有2例AT1-AA阳性(28.57%)。DM组AT1-AA阳性率明显高于N组(P<0.05);A组UPE、BUN及Scr水平均高于B组(P<0.01)。结论:自身免疫机制可能参与了DM肾损害的发生。Objective: To observe the role of the autoantibodies against the angiotensin II type 1 receptor(AT1-AA)in the development of diabetic nephropathy rats. Method: Thirty-six male Wistar rats of 8 weeks age were randomly divided into a normal control group (n=7) and a model group (n=29). Rats of the normal control group were fed with regular diet, while rats of the model group were fed with high-fat and high-sugar diet and 4 weeks later were given an intraperitoneal injection of 30mgKg streptozotocin (STZ). At 16th week, the rats were sacrificed, and serum autoantibodies against the AT1 receptor was measured by ELISA and the levels of 24-hour urine protein, serum creatinine and blood urea nitrogen were measured in all rats. The rats in model group with positive results of AT1-AA was defined as group A, the negative rats were group B. Results: 29 rats model of type 2 diabetic nephropathy were successfully constrcuted,which was distributed in group A(n=11,37.93%) group B(n=18 ,62.07%).The positive rate of AT1-AA was higher in DM group than in normal control group(28.57%,27)(P<0.05). Compared with the group B, the group A had significantly higher levels of urine protein, serum creatinine and blood urea nitrogen (P<0.05). Conclusion: This finding suggests that autoimmune mechanisms might play a role in the pathogenesis of diabetic nephropathy.

关 键 词：糖尿病肾损害 AT1受体 自身抗体 

分 类 号：R589[医药卫生—内分泌]