GK大鼠胃转流术后空腹血糖及骨骼肌SIRT3酶变化的实验研究  

Changes in fasting blood glucose and skeletal muscle SIRT3 enzyme in GK rats after undergoing gastric bypass operation

作　　者：丁洪亮[1] 黄宗海[1] 吴良平[2] 戴晓江[2] 

机构地区：[1]广州军区广州总医院普通外科,广东广州510010 [2]南方医科大学珠江医院普通外科,广东广州510282

出　　处：《海南医学院学报》2013年第2期145-147,151,共4页Journal of Hainan Medical University

基　　金：中国高校医学期刊临床专项资金项目(112210501)~~

摘　　要：目的:探讨GK大鼠行胃转流术后空腹血糖及骨骼肌SIRT3酶的变化及意义;方法:24只Goto-Kakizaki(GK)大鼠随机分为手术组(operation group OG组,假手术组(sim-operation group SG组)和对照组(control group CG组);OG组大鼠行胃转流手术,SG组大鼠行假手术,CG组仅作术前对照,OG组和SG组分别测定术前、术后48h和1、2及4周空腹血糖水平,术后4周处死取大腿部骨骼肌用western blot法半定量测定其SIRT3酶值;CG组监测空腹血糖水平,4周后处死同法测定大腿骨骼肌SIRT3酶值。结果:术前3组大鼠空腹血糖水平相仿,术后OG组血糖水平进行性下降,2周后血糖水平稳定,与SG组和CG组比较明显降低(P﹤0.05),SG组与CG组各时相空腹血糖水平差异无统计学意义(P﹥0.05);OG组术后骨骼肌SIRT3酶表达与明显增加,与SG组和CG组比较差异有统计学意义;结论:胃转流术可降低GK大鼠空腹血糖,骨骼肌SIRT3酶表达的增加可能是其糖尿病改善的机制之一。Objective： To investigate the changes m tasting blood glucose ane s^etetat muscle SIRT3 enzyme in GK rats after undergoing gastric bypass operation. Methods：Twenty-four Goto-Kakizaki （GK） rats were randomly divided into the operation group （OG group, n=8）, sham group （SG group, n m-8） and the control group （CG group, n-~8）. OG group were subjected to gastric bypass surgery, SG rats underwent a sham surgery, and CG group served as preoperative control. 48 h and 1, 2 and 4 weeks of fasting blood glucose levels of the OG group and SG groups were measured. Four weeks after the surgery, rats were sacrificed and the thigh skeletal muscle was taken out, semi-quantitative determination of SIRT3 levels were measured with western blot method. Results. The preoperative fasting glucose levels were simi- lar among the three groups, after surgery, glucose level of OG group declined, and achieved stable 2weeks later, showing significant difference from group SG and CG. （P^0.05）. No significant difference in fasting blood glucose levels was observed between group SG and CG （P^0.05）. The skeletal muscle SIRT3 enzyme expression level of the OG group were significantly increased compared with the SG group and CG group （P〈0.05）. 0onclusion. The gastric bypass can reduce fasting blood glucOse level in GK rats, increase in skeletal muscle SIRT3 enzyme expression might be one of the mechanisms by which dia- betes can be improved.

关 键 词：胃转流术 Goto—Kakizaki大鼠 SIRT3酶 

分 类 号：R587[医药卫生—内分泌]