罗格列酮对糖尿病大鼠肺组织中白细胞介素6和白细胞介素10表达的影响  

Effects of rosiglitazone on interlukin-6 and interlukin-10 levels in lung tissue of diabetic rats

作　　者：聂丽萍 穆维新[2] 沈亚欣[3] 郭庆军[3] 

机构地区：[1]河北省沧州市肃宁县医院内分泌科,062350 [2]河北医科大学第四医院急诊内科 [3]河北医科大学手术学和总论教研室

出　　处：《中国综合临床》2013年第3期225-228,共4页Clinical Medicine of China

基　　金：河北省医学科学研究重点课题（20120118）

摘　　要：目的观察罗格列酮对糖尿病大鼠肺组织中自细胞介素（IL）石、IL-10的影响。方法通过高糖、高脂饮食加腹腔注射小剂量链尿佐菌素（STZ）的方法，建立2型糖尿病大鼠模型，采用光镜动态观察10、20周时，对照组（8只）、单纯糖尿病组（8只）、罗格列酮治疗组（7只）大鼠肺组织的病理改变情况，并用免疫组织化学方法观察IL-6、IL-10的表达。结果对照组、单纯糖尿病组、罗格列酮治疗组10、20周IL-6分别为0．15±0．01、0．16±0．01与0．22±0．02、0．31±0．04和0．22±0．03、0．20±0．02；统计结果为F组内=216．89，P〈0．0l；F组间=342．62，P〈0．0l；F交互=341．51，P〈0．0l。除对照组10周与20周比较差异无统计学意义（P〉0．05）外，其他任意两组的比较差异均有统计学意义（P均〈0．05）。对照组、单纯糖尿病组、罗格列酮治疗组10、20周IL-10分别为0．13±0．01、0．15±0．02与0．20±0．01、0．21±0．01和0．20±0．02、0．17±0．01；统计结果为F相内=14．612，P〈0．01；F组间=909．19，P〈0．01；F交互=210．55，P〈0．01。对照组10周与20周IL—10比较差异无统计学意义，其他任意两组IL－10的比较差异均有统计学意义（P均〈0．05）。结论糖尿病大鼠肺组织中IL-6、IL－10升高可能是导致糖尿病大鼠肺部炎症损伤原因之一，罗格列酮可通过调控IL-6、IL－10来减轻糖尿病大鼠肺组织的炎症病理变化。Objective To observe the effect of rosiglitazone on the concentration of interlukin （ IL）-6 and IL-10 in lung tissues of diabetic rats. Methods The experimental diabetic rats were yielded by injecting streptozotocin（STZ） and feeding with high fat and high glucose food. We observed lung morphology in control group,diabetes mellitus（DM） group, and rosiglitazone group at 10 week and 20 week respectively under light microscope. Alteration of IL-6 and IL-10 in lung was measured by immunohistochemistry. Results The optical density values of IL-6 in the control group, the DM group and the roggerosiglitazone treatment group were 0. 15 ± 0.01,0.16±0.01;0.22 ±0.02,0.31 ±0.04;0.22± 0.03,and 0.20 ±0.02 at 10 week and 20 week respectively （ Fwithin = 216. 89, P 〈 0. 01 ; Fbetween = 342. 62, P 〈 0. 01 ; Finteraction = 341.51, P 〈 0. 01 ）. Any two groups had significant difference（P 〈 0. 05 ） except the comparison of the IL-6 values at 10 week and 20 week in the control group（ P 〉 0. 05 ）. The absorbance values of IL-10 in the three groups were 0. 13 ±0.01, 0. 15 ± 0. 02 ; 0. 20 ± 0. 01,0. 21 ± 0.01 ; 0. 20 ± 0.02, and 0. 17 ± 0. 01 at 10 week and 20 week respectively （Fwithin = 14. 612, P 〈 0. 01 ;Fbetween = 909. 19, P 〈 0. 01 ;F interaction = 210. 55, P 〈 0. 01 ）. Any two groups had significant difference（P 〈 0. 05 ） except the comparison of the IL-6 values at 10 week and 20 week in the control group. Conclusion The elevated levels of IL-6 and IL-10 in lung tissue of dibtetic rats might be related to the inflammation of lung tissues. Rosiglitazone may alleviate lung inflammation by regulating the levels of IL-6 and IL-10.

关 键 词：罗格列酮 糖尿病 白细胞介素6 白细胞介素10 

分 类 号：R587[医药卫生—内分泌]