花生蛋白激酶基因AhSTK的克隆与序列分析  

Cloning and Sequence Analysis of Protein Kinase Gene AhSTK from Arachis hypogaea L.

作　　者：朱岩[1] 彭振英[2] 范仲学[2] 张斌[2] 王成祥[1] 郑玲[3] 毕玉平[1,2,3] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]山东省农业科学院高新技术研究中心/山东省作物遗传改良与生态生理重点实验室,山东济南250100 [3]山东大学农学院,山东济南250014

出　　处：《山东农业科学》2013年第1期9-13,共5页Shandong Agricultural Sciences

基　　金：国家自然科学基金(30871541);国家国际科技合作专项项目(2012DFA30450);山东省济南市科技发展计划项目(201004044)

摘　　要：从优质花生鲁花14的cDNA文库中发现一条序列,全长为1 399 bp,3'端含有polyA尾,通过blastx搜索得知其为蛋白激酶基因,命名为AhSTK。以该花生cDNA序列为模板设计引物,克隆得到花生Ah-STK基因。序列分析表明,AhSTK基因的开放阅读框长度为1 080 bp,编码359个氨基酸,预测其分子量为38.9 kD,等电点为6.42,编码的蛋白质无信号肽,无跨膜结构,预测定位于细胞质。与其他植物中蛋白激酶的氨基酸序列比对发现,与大豆GmSTK蛋白同源性最高。器官特异性分析发现,该基因在花生根中表达量较高,而在花中表达量较低。花生AhSTK基因的克隆为进一步研究其生物学功能和应用奠定了基础。In this study, one new sequence total as 1 399 bp with polyA in 3＇ end was obtained from the cDNA library of peanut cultivar Luhua 14. By blastx analysis, it was identified as a protein kinase gene, named as AhSTK. With its cDNA sequence as template, the specific primers were designed to clone the Ah- STK gene. The sequence analysis indicated that the open reading frame of AhSTK gene had 1 080 bp bases, and encoding 359 amino acids with the molecular weight about 38.9 kD and the isoelectric point about 6.42. The encoded protein had no signal peptide and transmembrane domains, and was predicted to localize in cyto- plasm. Compared with the amino acid sequences of other plant protein kinases, AhSTK had the highest homol- ogy with that of soybean. Organ specificity analysis showed that its expression level was higher in peanut roots, but lower in flowers. The cloning and sequence analysis of AhSTK gene would lay foundations for further study of its biological functions and practical application.

关 键 词：花生 蛋白激酶 AhSTK蛋白 序列分析 器官特异性表达 

分 类 号：Q785[生物学—分子生物学]