利用回交转育培育黄淮稻区抗虫转基因水稻新品系  

Breeding Transgenic Rice Varieties with Insect Resistance for HuangHuai Area by Backcrossing

作　　者：柳絮[1] 王文英[1] 李军[1] 吴丹妮[1,2] 徐仅婷[1,2] 姚方印[1] 

机构地区：[1]山东省农业科学院高新技术研究中心,山东济南250100 [2]仲恺农业工程学院,广东广州510225

出　　处：《山东农业科学》2013年第1期38-41,45,共5页Shandong Agricultural Sciences

基　　金：国家转基因重大专项"粳稻种植区抗虫水稻新品种的培育"(2011ZX08001-001)

摘　　要：利用黄淮稻区主栽品种圣稻13、圣稻15、镇稻88为受体亲本,选择以籼稻明恢63为背景的转基因抗虫材料TT51(cry1Ab/1Ac)、T2A-1(cry2A)、T1C-19(cry1C)和以粳稻中花11为背景的转基因抗虫材料RJ-5(cry1C)为供体,分别进行杂交和多代回交。每一世代后代单株,通过涂抹Basta抗性筛选,结合PCR鉴定跟踪抗虫目的基因以及田间抗虫性鉴定、农艺性状选择,培育出来源于TT51带有cry1Ab/1Ac基因的抗虫稳定株系3个,来源于T2A-1带有cry2A基因的稳定株系2个,来源于T1C-19带有cry1C基因的稳定株系3个,来源于RJ-5带有cry1C基因的抗虫稳定株系2个。这些株系于田间均表现出很好的抗虫性状和优质丰产性状,为黄淮稻区抗虫转基因水稻育种奠定了基础。The donor parents, transgenic insect- resistant Indica rice Trs1 with crylAb/1Ac, T2A -1 with cry2A and TIC -19 with crylC from Minghui 63 and Japonica rice RJ-5 with crylC from Zhonghua 11 were transferred into the main acceptor parents Shengdao 13, Shengdao 15 and Zhendao 88 cultivated in Huan- gHuai area by successive backcrossing. Three lines from TFS1 with crylAb/1Ac,two lines from T2A -1 with cry2A,three lines from T1C - 19 with crylC and two lines from RJ - 5 with crylC were selected based on the Basra detection, PCR identification and field insect -resistance selection. These lines performed better insect -resistance and high -yielding characters in field, so it laid foundation for breeding transgenic rice varieties with insect resistance in HuangHuai area.

关 键 词：抗虫转基因水稻 回交转育 Basta PCR检测 抗虫性鉴定 

分 类 号：S511.035.3[农业科学—作物学]