高效液相色谱法测定莲胆消炎片中穿心莲内酯和脱水穿心莲内酯含量  

HPLC Determination for Andrographolide and dehydroandrographolide in Liandan Xiaoyan Tablets

作　　者：陆滢 

机构地区：[1]江苏省盐城市第一人民医院药剂科临床药学室,江苏盐城224000

出　　处：《中国药业》2013年第4期32-34,共3页China Pharmaceuticals

摘　　要：目的建立测定莲胆消炎片中穿心莲内酯和脱水穿心莲内酯含量的高效液相色谱法。方法采用Agilent C18柱(150 mm×4.6 mm,5μm);甲醇-水(52∶48)为流动相。穿心莲内酯的流量为0.8 mL/min,检测波长为225 nm;脱水穿心莲内酯的流速为0.8 mL/min,检测波长为254 nm;柱温30℃;进样量为10μL。结果莲胆消炎片中穿心莲内酯和脱水穿心莲内酯的进样量均在5～400μg范围内与峰面积呈良好线性关系,r=0.999 9(n=5),平均回收率为97.74%。结论该方法快速、简便、灵敏、准确,适合于莲胆消炎片中穿心莲内酯和脱水穿心莲内酯的含量测定。Objective To develop a HPLC method for the determination for andrographolide and dehydroandrographolide in Liandan Xiaoyan Tablets.Methods The Agilent C18 column（150 mm×4.6 mm,5 μm） was used with the mobile phase of methanol-water（52 ∶48）.The flow rate of andrographolide was 0.8 mL/min,the detection wavelength was 225 nm.The injected quantity was 10 μL.The flow rate of dehydroandrographolide was 0.8 mL/min,the detection wavelength was 254 nm and the column temperature was 30 ℃.The injected quantity was 10 μL.Results The linearity of the peak area was good when the injected quantity of andrographolide and dehydroandrographolide was in the range of 5-400 μg,（r=0.999 9,n=5）.The recovery rate of this method was 97.74%.Conclusion This method is rapid,convenient,sensitive and suitable for determining the contents of andrographolide and dehydroandro-grapholide in Liandan Xiaoyan Tablets.

关 键 词：高效液相色谱法 含量测定 穿心莲内酯 脱水穿心莲内酯 莲胆消炎片 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]