HPLC法测定五淋化石颗粒中迷迭香酸  

Determination of rosmarinic acid in Wulin Huashi granule by HPLC

作　　者：陈丽[1] 敬应春[2] 周银[1] 林艳芳 邱明丰[1] 赵应红 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海市中药研究所,上海201401 [3]西双版纳州民族医药研究所,云南景洪666100

出　　处：《国际药学研究杂志》2013年第1期105-107,共3页Journal of International Pharmaceutical Research

基　　金：云南省科技厅项目(2009BC014;2008IF018)

摘　　要：目的建立HPLC测定五淋化石颗粒中迷迭香酸含量的方法。方法以50%甲醇溶液超声提取制备供试品溶液,以Agilent Eclipse XDB-C18(4.6 mm×250 mm,5μm)为分析柱,乙腈-0.1%磷酸(21∶79)为流动相,体积流量1.0 ml/min,柱温30℃,检测波长330 nm。结果迷迭香酸在0.04～2.02μg范围线性关系良好(r=0.9999),回收率为97.81%,RSD为1.81%(n=9)。结论该方法简单、准确性和重点性良好,为五淋化石颗粒质量控制提供了可靠方法。Objective To establish a HPLC method for determination of rosmarinic acid in Wulin Huashi granule.Methods The determination was performed on a Eclipse XDB-C18 column with acetonitrile-0.1% phosphoric acid（21∶79） as the mobile phase.The flow rate was 1.0 ml/min,the detection wavelength was set at 330 nm,and the column temperature was set at 30℃.Results Rosmarinic acid presented a good linearity in the range of 0.04-2.02 μg（r=0.9999）.The average recovery was 97.81 % and RSD was 1.81%.Conclusion The method is simple,accurate and reproducible.It can be used for quality control of Wulin Huashi granule.

关 键 词：五淋化石颗粒 迷迭香酸 HPLC 含量测定 

分 类 号：R286.0[医药卫生—中药学]