HPLC测定大菟丝子中的咖啡酸含量和薄层鉴别  

Caffeic acid content by HPLC Cuscutaceae seed and TLC identification

作　　者：欧恒熙[1] 鲍家科 曹诗伟 刘玲[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004 [2]贵州省药品审评认证中心,贵州贵阳550004 [3]贵州神奇药业股份有限公司,贵州贵阳550004

出　　处：《中国民族民间医药》2013年第4期18-20,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:以咖啡酸为指标,研究并建立大菟丝子药材的质量标准。方法:以甲醇-0.1%磷酸(75∶25)为流动相,Diamo-sil色谱柱,流速1ml.min-1,在325nm处进行咖啡酸的HPLC测定。鉴别项采用薄层色谱法(TLC)为定性鉴别方法;结果:咖啡酸在HPLC进样0.3040～2.1280μg线性关系良好(0.9999),平均加样回收率为100.03%,RSD为0.2%(n=5)。TLC能检测出咖啡酸的特征斑点;结论:该方法简单可行,可用于大菟丝子药材的质量控制。定性定量方法快速、准确、专属性强、重现性好,可用于大菟丝子药材的质量控制。Objective： Take caffeic acid as index, study and establish the quality standard of Cuscutaceae seed. Method Methanol - 0. 1% phosphoric acid （75：25 ） was used as mobile phase. Detect caffeic acid content using HPLC absorbance of 325nm and velocity of flow was 1 ml . min^-1. The column used in this method was Diamosil. Result： the optical density had a good linear relationship with caffeic acid concentration at the rang： 0. 3040 ~ 2. 1280ug （R = 0. 9999）. The average recovery rate was 100. 03%, RSD 0. 2% （ n = 5 ）. Characteristic spots could be found in Thin -layer chromatogram. Conclusion： the method established in this study was relatively simple and can be applied for the quality controlment of Cuscutaceae seed.

关 键 词：大菟丝子 质量标准 薄层色谱法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]