红阳猕猴桃及其杂交后代的ISSR指纹图谱构建及遗传多样性分析  

Construction of ISSR Fingerprinting and Analysis of Genetic Diversity in Hongyang Kiwifruit and its of Hybrid Progenies

作　　者：谢玥[1] 潘美玲[2] 庄启国[1] 罗雪梅[1] 李明章[1] 

机构地区：[1]四川省自然资源科学研究院,成都610015 [2]四川农业大学园艺学院,雅安625014

出　　处：《基因组学与应用生物学》2013年第1期76-82,共7页Genomics and Applied Biology

基　　金：国家科技部项目"猕猴桃优良种质资源的发掘与杂交育种研究"(2009DFA30870);四川省国际科技合作与交流研究计划项目"猕猴桃抗溃疡病育种材料的筛选";四川省省属科研机构基本科研业务费项目资助

摘　　要：本文采用ISSR标记分析红阳（HY）及其杂交后代共14份材料的遗传多样性，并构建其ISSR指纹图谱。结果表明：从100条ISSR随机引物中筛选出7条引物，在14个供试材料中扩增出92条多态性条带，遗传相似系数在0．308-0．904之间；在相似系数水平为0．607时，可将所有供试材料聚为三类。本研究构建的指纹图谱可有效鉴别供试材料，为今后猕猴桃育种和杂交亲本的选择提供依据。The genetic diversity of the 14 materials including Hongyang kiwifruit varieties and their hybrid proge- nies were analyzed by using ISSR markers, and the fingerprinting profiles of these materials were constructed in this research. The results showed that 7 primers were screened out from 100 primers to apply to ISSR amplification. Total 92 polymorphic loci were detected. The similarity coefficients calculated by NTSYSpc were in the range from 0.308 to 0.904, and the tested 14 kiwi fruit materials were clustered into three groups at 0.607 of the similarity coefficient. Overall, the fingerprinting constructed in this study can effectively identify the test materials, which might provide a basis for selection of the hybrid parents and kiwifruit breeding program.

关 键 词：红阳猕猴桃 ISSR 指纹图谱 遗传多样性 

分 类 号：S663.4[农业科学—果树学]