拮抗菌与病原菌碳水化合物酶类比较分析  

Comparative Analysis of the Carbohydrate-active Enzymes between Antagonistic Microorganism and Plant Pathogen

作　　者：柳少燕[1] 陈捷胤[1,2] 李蕾[1] 戴小枫[1,2] 

机构地区：[1]中国农业科学院农产品加工研究所,北京100193 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《基因组学与应用生物学》2013年第1期97-104,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(31171799);国家"973"计划项目(2011CB100700)资助

摘　　要：拮抗菌通过分泌细胞壁降解酶降解病原菌细胞壁组分是其重要的拮抗方式。本研究以2个拮抗菌(绿色木霉和枯草芽孢杆菌)和7个植物病原菌为研究对象,对碳水化合物酶类CAZymes(Carbohydrate-active enzymes)进行了注释和比较分析,明确了拮抗菌相对于病原菌发生扩增的细胞壁降解酶亚家族;利用ClustalX2.0多序列比对、Mega4.0构建系统发育树和MEME软件预测保守基序的方法,分析了扩增亚家族的序列结构和进化特征。结果表明,拮抗菌中可能参与病原菌细胞壁降解酶类的亚家族CBM50、GH25和GH73发生了显著扩增,并通过序列比较和进化分析初步明确了扩增的亚家族与拮抗菌特异降解病原菌细胞壁组分之间存在关联,为拮抗菌细胞壁降解酶类亚家族CBM50、GH25和GH73参与拮抗的分子机理提供理论依据。The important way of antagonistic mechanism in antagonistic microorganisms is to degrade the pathogen cell wall by secreted cell-wall related enzymes. In this research, the CAZymes of two antagonistic microorganisms and seven pathogens were annotated with CAZymes database, and then identified the expansion CAZymes subfamilies in antagonistic microorganisms compared to pathogens. Moreover, the sequences characteristic and phylogenetic of expansion CAZymes subfamilies were analyzed by ClustalX 2.0, Mega4.0 and MEME softwares. The results showed that the cell wall degradation subfamilies of CBM50, GH25 and GH73 expanded in antagonistic microorganisms compared to pathogens, and these expansion subfamilies were close relatively to the specific degradation of pathogen cell wall. It is suppose that the CBM50, GH25 and GH73 subfamilies of antago- nistic microorganisms play an important role in the antagonistic mechanism to pathogens.

关 键 词：生物防治 拮抗菌 病原菌 细胞壁降解酶 基因家族扩增 

分 类 号：S476[农业科学—农业昆虫与害虫防治]