白菜根肿病菌拮抗微生物的筛选及防治效果评价  

Screening of Antagonistic Microorganisms against Plasmodiophora brassicae and Evaluation of Their Control Effect

作　　者：孙良菲[1] 任佐华[1] 彭沙莎[1] 黄小莉[1] 董俊[1] 刘二明[1] 

机构地区：[1]湖南农业大学生物安全科学技术学院,湖南长沙410128

出　　处：《湖南农业科学》2013年第1期84-87,共4页Hunan Agricultural Sciences

基　　金：公益性行业(农业)科研专项(201003029)

摘　　要：为了筛选对白菜根肿病菌具有拮抗作用的微生物,从湖南和云南等地采集了21份感病土样,并对其进行了分离筛选和防治白菜根肿病效果评价。结果表明:在21份感病土样中分离到471株菌株,其中细菌236株、真菌107株、放线菌128株。利用7种指示菌进行平板对峙测定,从中筛选出17个抗菌谱广的菌株。采用地衣红染色法测定这17个菌株对白菜根肿病菌休眠孢子萌发的抑制效果,筛选出了放线菌YN-6、真菌XP-F2,其对白菜根肿病菌休眠孢子抑制率分别为61.75%、56.84%。温室盆栽试验结果显示,放线菌YN-6、真菌XP-F2对白菜根肿病的防病效果分别为56.30%、61.80%,对白菜根肿病具有一定的生防潜力。To screen antagonistic microorganisms against Plasmodiophora brassicae,21 diseased soil samples were collected from Hunan Province and Yunnan Province,and then be isolated to screen antagonistic microorganisms against Plasmodiophora brassicae.Moreover,the control effect of screened antagonistic microorganisms was evaluated.The results indicated that a total of 471 strains,among which 236 bacteria,107 fungi and 128 actinomycetes strains were screened from the 21 diseased soil samples.17 strains were screened from the above 471 strains using the dual culture method with seven species of indicator plant pathogenic fungi.Then,the inhibition effect of the 17 strains against germination of resting spores of Plasmodiophora brassicae was determined by orcein staining method.The results showed that the inhibition rate of actinomycete YN-6 and fungus XP-F2 on the germination of resting spore of Plasmodiophora brassicae was 61.75% and 56.84%,respectively.In addition,the results of greenhouse pot experiment showed that the controlling efficacy of YN-6 and XP-F2 on cabbage clubroot was 56.30% and 61.80%,respectively.So they may have certain capacity for controlling Chinese cabbage clubroot.

关 键 词：白菜根肿病 拮抗微生物 休眠孢子 防治效果 

分 类 号：S436.34[农业科学—农业昆虫与害虫防治]