一种适合检测养殖场环境中链球菌的PCR检测方法  

The rapid detection assay for the muramidase-released protein of streptococcus suis in the environment around the farm

作　　者：王秋实[1] 曹冶[1] 谢晶[1] 赵素君[1] 于吉锋[1] 罗丹丹[1] 叶勇刚[1] 李兴玉[1] 李江凌[1] 魏甬[1] 廖党金[1] 

机构地区：[1]四川省畜牧科学研究院,成都610066

出　　处：《广东畜牧兽医科技》2013年第1期30-33,共4页Guangdong Journal of Animal and Veterinary Science

基　　金：四川生猪现代产业技术体系岗位专家;四川省财政基础科研经费专项;国家支撑计划项目(2012BAD12B03);四川省支撑计划项目(2010NZ0106)

摘　　要：根据溶菌酶释放蛋白的编码序列特点设计引物,以建立养殖场环境中链球菌的PCR快速检测方法。结果在链球菌参考株都扩增到885 bp特异性目的片段,而肠毒素性大肠杆菌、沙门氏菌、金黄色葡萄球菌等均不能检出;10倍系列稀释结果显示,本方法敏感性可达到检测单个菌株;对处理环境样品时,能检测到4×103CFU/g的环境链球菌。该方法具有简单、快速、准确的特点,大大缩短了检测环境中致病性猪链球菌的时间。A rapid PCR detection method of Streptococcus pollution in the environment around the farm was established based on the unique nucleotides sequence of the Muramidase-Released Protein （MRP）.A 885bp fragment is detected in the positive Streptococcus suis. strains, negative in the Enterotoxigenic E.coli ,Salmonella, Staphylococcus aureus etc.The sensitivity of this method can achieve a single germ in 10-fold dilution.4 × 103 CFU/g Streptococcus of the environment sample can be detected using this method. This method is simple, rapid, accurate, and greatly shorten the time of the detection of Streptococcus suis in the environment.

关 键 词：溶菌酶释放蛋白 链球菌 快速检测 

分 类 号：S852.611[农业科学—基础兽医学]