参龙汤对脑缺血大鼠血清炎性因子的影响  

Influence of Shenlong Decoction on Inflammatory Cytokines in Blood Serum with Chronic Cerebral Ischemia Rats

作　　者：陈利平[1] 王发渭[1] 孙志高[2] 许成勇[2] 黄泉智[2] 

机构地区：[1]解放军总医院海南分院,海南三亚572004 [2]解放军总医院,北京100853

出　　处：《中国中医急症》2013年第2期201-202,213,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：北京科委科技计划研发攻关基金(Z111107067311022)

摘　　要：目的观察参龙汤对脑缺血大鼠血清炎性因子白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、细胞间黏附分子1(ICAM-1)、肿瘤坏死因子(TNF-α)的影响。方法利用双侧颈总动脉结扎的方法制备慢性脑缺血模型,50只大鼠分为:对照组、模型组、脑复康组、大剂量参龙汤组及小剂量参龙汤组。药物处理4周后,采用ELISA法检测各组大鼠血清IL-6、IL-8、ICAM-1、TNF-α含量的变化。结果脑缺血模型组大鼠的血清IL-6、IL-8、ICAM-1和TNF-α的含量较对照组显著升高。大剂量参龙汤组可显著降低血清中IL-6、IL-8、ICAM-1、TNF-α的含量,较模型组明显降低。结论参龙汤大剂量组可显著降低血清中IL-6、IL-8、ICAM-1、TNF-α的含量,减少血清中炎性因子。这可能是参龙汤对脑缺血神经损伤的保护作用机制之一。Objective： To investigate the effect of Shenlongtang（SLT） decoction on the contents of tumor necrosisfactor-α（TNF-α） and interleukin-6（IL-6）,interleukin-8（IL-8）,intercellular adhesion molecule-1（ICAM-1） in blood serum of rats with cerebral ischemia model.Methods： The cerebral ischemia animal model was established by two-vessel occlusion.50 rats were randomly divided into 5 groups： control group with slam operation;model group;positive control group of pisacetam;SLT groups（low and high dosage）.Four weeks later,The contents of TNF-α,IL-6,IL-8,ICAM-1 in blood serum were determined by enzyme linked immunosorbent assay（ELISA）.Results： The contents of TNF-α,IL-6,IL-8,ICAM-1 in blood serum were significantly lower in the sham operation group than those in the cerebral ischemia group.The contents of TNF-α,IL-6,IL-8 and ICAM-1 in blood serum were significantly lower in the SLT high dosage group than those in the cerebral ischemia group.Conclusion： SLT has neuron protective effect by down regulation of the contents of the inflammatory cytokines in blood serum with cerebral ischemia rats.

关 键 词：参龙汤 脑缺血 白细胞介素-6 肿瘤坏死因子-Α 细胞间黏附分子1 

分 类 号：R285.5[医药卫生—中药学]