液相色谱法测定水中苯脲类除草剂  

Determination of Phenylurea Herbicides in Water by Liquid Chromatography

作　　者：李向梅[1] 付艳芳[1] 周建科[1] 

机构地区：[1]河北大学理化分析中心河北省分析科学技术重点实验室,河北保定071002

出　　处：《现代农药》2013年第1期37-39,43,共4页MODERN AGROCHEMICALS

摘　　要：采用自制微萃取瓶富集,高效液相色谱法测定水中痕量灭草隆、绿谷隆、溴谷隆和敌草隆。以800 L正辛醇萃取300 mL水样,使用反相C18色谱柱,流动相为甲醇+水+乙腈(体积比为60∶38∶2),245 nm紫外检测。采用基质校正工作曲线,在优化条件下,4种除草剂在5.0～100.0 ng/mL范围内线性良好,相关系数为0.998 0～0.999 4,相对标准偏差(RSD)为2.0%～3.4%。A method based on enrichment with microextraction flasks and high performance liquid chromatography was used for determination of phenylurea herbicides,such as HHmonuronHH,HHmonolinuronHH,metobromuron and HHdiuronHH in water.These herbicides were separated on a C18 column（250 mm×4.6 mm,5 μm） employing methanol,water and acetonitrile（the volume ratio of 60∶38∶2） as mobile phase and determined with UV detector at 245 nm.800 μL HHn-octylHH HHalcoholHH was used as extractant to extract 300 mL water.Under the optimal conditions,the linearities of regression equations were good in the range of 5.0-100.0 ng/mL with the correlation coefficients of 0.9980-0.9994.The relative standard deviations（RSD） of four herbicides were 2.0%-3.4%.

关 键 词：微萃取瓶 液相色谱 苯脲类除草剂 灭草隆 绿谷隆 溴谷隆 敌草隆 

分 类 号：X839.2[环境科学与工程—环境工程] O657.72[理学—分析化学]